Table 4. The MutaBind2 mutation-induced change in binding affinity prediction. The input requires the PDB structure of the protein complex with a minimum of 2 distinct chains. A negative and positive ΔΔGbind (kcal/mol) value correspond to stabilizing and destabilizing mutations, predicted to increase and decrease the binding affinity respectively. An all-positive value for the MutaBind2 output denote a destabilizing effect on the protein-protein interaction.