Cell Line and Expansion
A GS CHO cell line expressing a proprietary monoclonal antibody (mAb) was used for this study. The seed train was initiated from a vial thaw and scaled up in shake flasks to generate enough cells for production bioreactors. Cell expansion was carried out in CD CHO medium (Gibco, NY) and routinely passaged to maintain exponential growth. The shake flasks were maintained in shaking incubators at 36.5°C, 5% CO2, 70% humidity, and 100 RPM.