2.6 Preparation of DNA, PCR amplification of 16S rRNA genes, and Sanger sequencing
For the phylogenetic analyses of bacterial isolates, genomic DNA was extracted from the cells of the isolates and purified by the method of Saito and Miura (1963). PCR amplification of 16S rRNA genes with primers 27F and 1492R, and sequencing of the PCR products were performed as described previously (Iino et al. , 2015a).