2.2 Specimens for bacterial isolation
Crude oil emulsion samples were collected from an oil-production well in Akita Prefecture and two crude oil storage tanks, one in Kagoshima Prefecture and the other in Miyagi Prefecture, Japan. A corrosion-scale sample was collected from the inner surface of a cast-iron pipe for brine transportation at a natural-gas- and iodine-production plant in Chiba Prefecture, Japan. Each of these samples was kept in a transparent oxygen-barrier plastic bag containing an AnaeroPack-Anaero sachet (Mitsubishi Gas Chemical) until inoculation in fresh media.