Establishment of DNFB induced inflammation model of the
dorsal skin in mice
The hair of mice was shaved firstly, and then the remaining hair was
removed with depilatory cream. The interval of one day is recorded as
the day 0 and is not processed. At day 1, 100 μl 0.5 % DNFB was
topically applied to sensitize the prepared dorsal skin of mice. No DNFB
treatment at day 2, 3 and 4. At day 5 and day 6, 50 μl 0.2 % DNFB was
applied to challenge the skin respectively. The solvent (acetone/olive
oil, 4:1) was used as a control.