Chromosome Pathway Analysis
In order to compare the gene composition in regions of interest, we performed statistical gene enrichment analysis using the online version of g:Profiler (version e102_eg49_p15_7a9b4d6) with the g:SCS multiple testing correction method applying a significance threshold of 0.05 (Raudvere et al., 2019). This tool includes a database of Gene Ontology (GO) (Ashburner et al., 2000; Gene Ontology Consortium, 2021) and Kyoto Encyclopedia of Genes and Genomes (KEGG) (Kanehisa & Goto, 2000) functional annotations based on the draft male mountain pine beetle genome (DendPond_male_1.0). We used BLASTn with the gene models identified in the final female assembly to identify the corresponding gene IDs in this draft genome for analysis.