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Figure 1 Map of the approximated limits of pre-Illinoian (2.5 - 0.5 million years ago), Illinoian (300,000 - 140,000 years ago), and Wisconsinan (19,000 - 10,500 years ago) glacial advancements in the central portion of North America.
Figure 2 The approximate geographic distribution of the rainbow darter (Etheostoma caeruleum ) in eastern North America: spatial data obtained from NatureServe (2013). Insets indicate sampling localities within the Volga and Meramec rivers. Arrows indicate direction of flow of water. Image of a male rainbow darter.
FIGURE 3 Estimated membership coefficients based on admixture analyses. Vertical axis indicates membership coefficient and the horizontal axis indicates the locality for each individual. A. Combined river dataset for K = 4, B. Volga-only dataset for K = 4 and C. Meramec-only dataset for K = 3.
FIGURE 4 Scatterplots from discriminant analysis of principal components (DAPC) with individuals designated by locality. Eigenvalues of the analyses are displayed as insets. A. Combined river dataset. B. Volga-only dataset. C. Meramec-only dataset.
FIGURE 5 Scatter plots of the Mantel tests for isolation by distance using in-river distances with the regression line (red) and Lowess smoothed regression / trend line (dashed blue). A. Volga-only dataset (p = 0.259, R2 = 0.245) and B. Meramec-only dataset (p = 0.015, R2 = 0.904).