Note: Reactions were performed in triplicate with
resting cells of WT, W1, W2, or S3 (10 g/L wet cells) and 13 L-amino
acids (100 g/L; Leu, L-Ile, L-Met, L-Val, L-Ala, L-Phe, L-Trp, L-Tyr,
L-Arg, L-Asp, L-Glu, L-Ser or L-Thr) in 20 mL Tris-HCl buffer (20 mM, pH
8.0) at 200 rpm and 25 oC for 24 h. The abbreviation
C. stands for conversion. The percentages in parentheses are the fold
change in conversion rate of the variant compared to the parentalPmi LAAD, and only the data that are significantly elevated are
indicated.