2.6. Enayme-linked immunosorbent assay
The CAR-T cells and target tumor cells were co-cultured at various effector/target (E:T) ratios in a 96-well plate for 24hr at 37℃. The culture supernatants were harvested and subjected to ELISA to detect INF-γ and Granzyme B, according to the manufacturer’s instructions. Every value represented the mean of triplicate wells.