2.2. Cell lines and cell culture conditions
SW480, HCT116, K562 and huh7 were obtained from ATCC. Human Jurkat cell line was maintained in our laboratory. These cells were cultured in RPMI 1640 medium (Gibco, Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS) (Biological Industries, ISR). Human breast cancer cell lines MDA-MB-231 and HS578T were obtained from ATCC and cultured in Dulbecco’s modified Eagle medium (Gibco, Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS) (Biological Industries, ISR). The HEK-293T cell lines were purchased from ATCC and maintained in complete growth medium (Gibco invitrogen), containing with 10% fetal bovine serum (FBS) (Biological Industries, ISR), 2mM L-glutamine (Gibco, Invitrogen) and 1mM sodium pyruvate solution (Gibco, Invitrogen). All above cells were cultured in a humidified atmosphere containing 5% CO2 at 37℃.