2.3. Human CAR-T cells generation
Human peripheral blood mononuclear cells (PBMCs) samples were obtained from healthy donors according to the manufacturer’s protocol. Written informed consents were obtained from all donors. PBMC were stimulated with anti-CD3/CD28 for 48 h. Then, the activated T cells were infected with the CAR-encoding lentiviral supernatant. 24h after lentiviral infection, the lentiviral supernatant was replaced with fresh medium containing RPMI-1640 and supplemented with 10% FBS (Gibco), 200U/ml IL-2, 25 mM HEPPES, 55 μM 2-M, 100 U/ml penicillin and 100 μg/ml streptomycin. The infected T cells were cultured for 7 days.