Colposcopy procedures
The involved patients were randomly divided into fluorescence colposcope group and conventional colposcope group by random number table method. Neither the registrar nor colposcopy operator participated in the random grouping work. For patients of fluorescence colposcope group, 0.1% fluorescent dye TMTP1-PEG4-ICG was apply to the cervix 30 minutes before colposcopy as we described previously8. 2-4 cervical sites with obvious fluorescent signal or suspected lesions were sampled for biopsy. For patients of conventional colposcope group, firstly, a cotton ball containing 5% acetic acid were applied to the cervix for one minute. Take out the cotton ball, observe for 2-3 minutes, and acquire acetic acid acetowhitening response pictures. Then wipe the cervix with a dry cotton ball and stain the cervix with 5% Lugol’s iodine solution. Observe for 1-2 minutes and collect the cervix images again. 2-4 cervical sites with abnormal visual changes or suspected lesions were taken for biopsy. If no suspicious lesions were observed in both of the groups, the 3, 6, 9, 12 areas of the cervix were sampled.