Fluorescence colposcope
The dual modal fluorescence
colposcope was developed by integrating a NIR fluorescent imaging system
into a conventional clinical colposcope (3ML LED, Leisegang, Berlin,
Germany) in our previous research8. The wavelength of
the excitation light source was consistent with the absorption peak of
TMTP1-PEG4-ICG, which was 785nm. The color reflectance imaging and NIR
fluorescence imaging of the cervix can be obtained simultaneously with
the dual modal fluorescence colposcope by a visible-NIR camera. The
magnification of 7.5×/15×/30× was provided, with the corresponding field
of view was 35.0mm/16.4mm/8.6mm and the corresponding minimum
discernible size was 70.1um/35.0um/24.8um. The working distance was
300mm, which ensured non-invasive operation. Besides, a software
integrated with graphical user interface (GUI) was developed to
facilitate the acquisition of the cervical images. The fluorescence
image was overlapped on the color reflectance image for the convenience
of spatial localization of the fluorescence signal. The color
reflectance images, fluorescence images and the merge images were
displayed simultaneously. The fluorescence colposcope possessed the
advantages of real-time imaging, high resolution, high SBR and
user-friendly control.