Fig. 2: (A) Measurement setup for measuring cellular impedance, (B)
measuring interdigitated electrodes (IDEs). Optical microscopic images
of (C) the MSCs on collagen thin films of 250μg/mL concentration and (D)
the HUVECs on collagen thin films of 500μg/mL concentration.
Statistical Analysis
Obtained results were analyzed utilizing SPSS 22 software. Data were
indicated as means ± standard error of the mean. All data were expressed
as the mean of three repeats. At each repeat of every concentration, at
least three samples were measured. The means were compared by student’s
T-test. Discrepancies were evaluated to be statistically significant at
p < 0.05.
Results and discussion
Effect of concentration of collagen thin films on the
viability of the MSCs cells
The MSCs were cultured in chambers on top of the IDEs modified with a
self-assembled monolayer (SAM) of 1-hexadecanethiol. After 48 h, cell
viability was detected via the dye exclusion method using trypan blue
staining. The obtained results are shown in Fig. 3 (A) for different
concentrations of collagen thin films. As shown in the diagrams, there
is no substantial difference among the viability of MSCs cells cultured
on the collagen thin films with various concentrations.