3.4. LASSO probes sensitivity assessment
In order to model the feasibility of a highly sensitive and selective
capture of a single gene in a background of the human genome, we
performed a series of capture reactions where we spiked consecutive
tenfold dilutions of the 7250 bp M13mp18 viral DNA in a constant human
genomic DNA background (1.6 fM, correspondent to 50 ng/µl) to a final
0.34 fM correspondent to 25*10-6 ng/µl. The captures
were performed by using tenfold dilutions of the new LASSO M13 probe
that was designed to capture 1kb target sequence within the M13mp18
genome. As shown in the Fig. 5 a , The expected capture band was
observed even when testing the lowest 0.35 fM target molar concentration
that was ~4 times lower than the Human DNA molar
background (~1.6 fM) in the capture reaction.