Figure 5. LASSO capture sensitivity assessment, a . Gel electrophoresis of post capture PCR amplicons obtained by capturing a single 1kb target sequence within the M13mp18 viral DNA (red) that was spiked according to five tenfold dilutions (3.5 pM, 350fM, 35fM, 3.5fM, 0.35fM) in a constant 1.6fM total Human genomic DNA background (green). Captures displayed the 15 lanes were performed by testing three tenfold dilutions of the LASSO probe (blue) of 72pM, 7.2pM and 720 fM respectively. - indicates negative control for the filling mix in the capture reaction. b . Post capture PCR of circles obtained from the capture of β -actin and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Lanes 6 capture of g-Actin, 7 capture of GAPDH, 8 and 9 negative control same as 6 and 7 but no LASSO library in the capture, 10 Positive control for capture reaction (1kb target within M13mp18 ssDNA. L indicates 1kb plus DNA ladder (NEB).