Figure 5. LASSO capture sensitivity assessment, a . Gel
electrophoresis of post capture PCR amplicons obtained by capturing a
single 1kb target sequence within the M13mp18 viral DNA (red) that was
spiked according to five tenfold dilutions (3.5 pM, 350fM, 35fM, 3.5fM,
0.35fM) in a constant 1.6fM total Human genomic DNA background (green).
Captures displayed the 15 lanes were performed by testing three tenfold
dilutions of the LASSO probe (blue) of 72pM, 7.2pM and 720 fM
respectively. - indicates negative control for the filling mix
in the capture reaction. b . Post capture PCR of circles
obtained from the capture of β -actin and Glyceraldehyde 3-phosphate
dehydrogenase (GAPDH). Lanes 6 capture of g-Actin, 7 capture of GAPDH, 8
and 9 negative control same as 6 and 7 but no LASSO library in the
capture, 10 Positive control for capture reaction (1kb target within
M13mp18 ssDNA. L indicates 1kb plus DNA ladder (NEB).