Massively parallel DNA target capture using Long Adapter Single Stranded Oligonucleotide (LASSO) probes assembled through a novel DNA recombinase mediated methodology
Lorenzo Tosi1, Lamia Chaikban1, Benjamin Larman2, Jeffrey Rosenfield3,4, Biju Parekkadan1,3*
1 Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey 08854, USA
2 Institute of Cell Engineering, Division of Immunology, Department of Pathology, Johns Hopkins University, Baltimore, MD, USA
3 Cancer Institute of New Jersey, New Brunswick, New Jersey 08854, USA
4 Department of Pathology, Robert Wood Johnson Medical School, New Brunswick, NJ 08903, USA
*Correspondence and requests for materials should be addressed to B.P. (biju.parekkadan@rutgers.edu; 599 Taylor Road, Piscataway, NJ 08854)
Key words : PCR, LASSO probe, Cre-Lox, DNA target capture
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