Figure 1, Schematic of starting components for DNA
recombinase mediated LASSO probe synthesis. a, pre-LASSO probe
that contains the ligation and extension arms (~25bp)
identical to the 5’ and 3’ ends of the targeted DNA sequence (ORF).b, pLASSO vector that contains two loxp sites oriented in the
same direction, origin of replication, ampicillin resistance and primer
annealing sites for linearization. c, Final Mature ssDNA LASSO
probe. Ligation and extension arms are linked to the Backbone sequence
(blue) that was obtained from pLASSO by a series of processing
reactions.