3.4 | FISH and GISH analysis
To further explore the genomic constitutions of natural hybrids, we
selected some hybrids for in situ hybridization.
St2-80 was a FISH marker for the St genome
(Wang, et al., 2017). Signals produced by St2-80 were
present on the entire arm of the St genome chromosomes, except
at the centromeric region and near centromeric region (Wang et al.,
2017). This marker was used to detect
if the St genome presented in the putative parents and hybrids.
FISH results showed that 14
chromosomes
of putative parents and hybrids displayed fluorescent signals indicating
that they contained one set of St genome (Figures 6a, c, e and
7a, c, e). This result was confirmed by GISH analysis, where 28
chromosomes of putative parents and hybrids were hybridized with theStY probe from R. ciliaris (Figures 6b, d, f and 7b, d,
f). The results of FISH and GISH indicated that the genomic constitution
of putative parents and 11 hybrids (RH1-3, RH1-8, RH1-11, RH1-14, RH2-2,
RH2-10, RH2-12, RH2-15, RH2-17, RH2-37, RH2-39) were StY .