Single copy nuclear gene is inherited from both parents and is rarely
affected by concerted evolution. Therefore, it is used as an ideal DNA
sequence in the analysis of plant molecular phylogeny to study the
relationship of related species, polyploid plant phylogenetic
relationship, speciation history of allopolyploid, source of species
genome donor, generation of hybridization events, and gene introgression
of polyploid species have significant advantages (Sang, 2002; Tang et
al., 2017). In the present study, we performed phylogenetic analyses of
the hybrids, the associated species (R. stricta, R.
turczaninovii, R. grandis, E. sibiricus) and other
Triticeae species with different genomic compositions. Two distinctDMC1 copies of the sequences from hybrids, R. stricta,R. turczaninovii and the other Triticeae species growing nearby
were divided into St and Y clades (Figure 8). In theSt clade, 15 hybrids sequences and R. turczaninoviiformed a subclade (BS=51%, PP=1.00). In the Y clade, 15
hybrids sequences, R. turczaninovii and R. stricta formed
a subclade (BS=64%, PP=0.83). The results of phylogenetic analysis
based on nuclear gene DMC1 showed that natural hybrids have close
relationships with R. stricta and R. turczaninovii,
and they all contained St and Y genomes. The results
were consistent with cytological studies.
Phylogenetic analyses based on rps16 sequence showed 5 hybrids
RH1 sequences were grouped with R. stricta (BS=64%, PP=0.97), 10
hybrids RH2 sequences were grouped with R. turczaninovii(BS=87%, PP=1.00). Furthermore, hybrids RH1 were collected fromR. stricta, hybrids RH2 were collected from R.
turczaninovii. So, R. stricta was the female parent of the
hybrids RH1, R. stricta was the female parent of the hybrids RH2.
Therefore, based on the results of
cytological and phylogenetic analysis, we suggested that R.
stricta and R. turczaninovii were the female and male parents of
the hybrids RH1; R.
turczaninovii and R. stricta were the female and male parents of
the hybrids RH2.