2.1 | Plant materials
57 putative nature hybrids including 17 hybrids (named RH1) found inR. stricta field and 40 hybrids (named RH2) appeared in R. turczaninovii field were used in this study (Table 1). The possible parents R. stricta and R. turczaninovii , and the other Triticeae species growing nearby were also obtained, including species of Roegneria and Elymus . All of them were collected from the experimental field of SAGS, Hongyuan County, Sichuan, China. 20 diploid species (representing the genomes St ,H , Ee ,Eb , W , P , Ta ,V , Ns , A , B , and D ), and species with different genomic combinations (StY , StH ) from the tribe Triticeae were used for DNA sequences analysis. The names of the sampled taxa, abbreviations, accession numbers, ploidy level, genomic constitution and GenBank accession numbers were listed in Table S1. Materials with PI and W6 were kindly provided by American National Plant Germplasm System (Pullman, WA, USA). The authors of the present study collected all other accessions, for which voucher specimens were deposited with the perennial nursery and herbarium of the Triticeae Research Institute, Sichuan Agricultural University, China (SAUTI).