5. Describe quality of template(s)
Higher resolution structures are preferred if a choice can be made between otherwise equally relevant structures. In the case of NMR structures, use stereochemical and other quality estimates. The regions most important for the intended use of the model should be reliably determined. Although the descriptions of templates can be found from articles and databases when using published structures, describe quality measures relevant for your choice and model application. If template is not published, even more specific descriptions are needed. In that case, include also the coordinates and description of the structure determination.
Sequence similarity and identity between the template and the protein to be modelled protein sets limits for quality of the model. If several related structures from different organism or for paralogs are available, they can provide additional evidence for the modelled structure.
Example. The template structure has been determined on high resolution (1.45 Å) and it shows good quality parameters (e.g. Rfree is 0.195). B values are low (<30) for the functionally relevant substrate binding region and catalytic site and secondary structural elements in the protein core. PROCHECK (Laskowski et al., 1993) report did not indicate any quality issues.