3.Results:
Partial laryngectomy was performed in 37 and total laryngectomy in 63
cases. Ninety-five cases were male, 5 were female, and the median age of
all cases was 63 (min: 43-max: 84, SD: 9.72) years. Sixty –one patients
survived, and 38 cases exited, while survival information of one case
was not available. The median OS time of the cases was 35.8 months (min:
2-max: 118, SD: 24.7).
Sixty–five glottic, 22 supraglottic, 1 subglottic, and 12 transglottic
tumors were examined The average size of the tumors was 2.64 cm (min:
0.60 cm-max: 8cm, SD: 1.35). Distribution of pathological stages of the
tumors was as follows: stage 1; n=22, stage 2; n=6; stage 3; n=22, stage
4A; n=35: and stage 4B; n=15. LNM was detected in 34 cases.
Histopathological examination revealed LVI in 25, PNI in 22 and necrosis
in 30 of the cases. The data are summarized in Table 1.
Immunohistochemically (IHC) E-cadherin was overexpressed in 93 and
downregulated in 7 tumors. Membranous staining was observed in 82,
cytoplasmic and membranous staining in 16, and only cytoplasmic staining
in 2 cases. Overexpression of beta-catenin was observed in 60, and its
downregulation in 40 cases. Membranous staining was seen in 29,
cytoplasmic and membranous staining in 66, and only cytoplasmic staining
in 5 cases. Staining intensity with N-cadherin was scored as follows:
Score 1 in 93, score 2 in 6, and score 3 in one tumor sample. Nuclear
staining was noted in 18, cytoplasmic staining in 14, cytoplasmic and
membranous staining in 1 case, while no staining was observed in 67
cases. Zeb1-negativity was detected in 91, and Zeb1-positivity in 9
tumor cells.
Percentage of Zeb1 positivity in the stroma surrounding the tumor was
also evaluated. Accordingly, Zeb1 positivity was observed below 10% in
19, between 10-50% in 48, and over 50% in 33 cases. The density of
fibroblasts in the surrounding stroma was scored using αSMA.
Accordingly, the staining density was ≤ 10% in 19, between 10-50%, in
48 and and above 50% in 41 cases.