Murine resident peritoneal macrophage:
Peritoneal exudate cells were harvested in ice-cold PEC harvest buffer
(PBS, 5 mM EDTA), cells were then pelleted and resuspended in DMEM
(containing 4.5 g·L−1 glucose, 2 mM
l‐glutamine, 50 units·ml−1 penicillin and 50
μg·ml−1 streptomycin, 10% heat‐inactivated FBS and
allowed to attached to tissue culture treated plastic for 1 h.
Unattached cells were washed off and cells stimulated as per
experimental protocol.