Murine resident peritoneal macrophage:
Peritoneal exudate cells were harvested in ice-cold PEC harvest buffer (PBS, 5 mM EDTA), cells were then pelleted and resuspended in DMEM (containing 4.5 g·L−1 glucose, 2 mM l‐glutamine, 50 units·ml−1 penicillin and 50 μg·ml−1 streptomycin, 10% heat‐inactivated FBS and allowed to attached to tissue culture treated plastic for 1 h. Unattached cells were washed off and cells stimulated as per experimental protocol.