Statistical analysis:
The data that support the findings of this study are available from the
corresponding author upon reasonable request. Some data may not be made
available because of privacy or ethical restrictions. All experiments
were designed, where possible, to generate groups of equal size. Power
calculations were used to estimate the group size based on an expected
effect size of 30%. Where possible, blinding and randomisation
protocols were used. All data in the text and figures are presented as
mean ± standard error mean (SEM) of n observations, wheren represents the number of animals studied (in vivo ) or
independent values, not technical replicates (in vitro ).
Exclusion criteria was pre-determined before the beginning of
experiment, if there was blood in the peritoneal at harvest an
individual n would be excluded, or at analysis stage outliners were
defined as greater than +/- 2 SD from the mean. For western blot data,
all data are represented as fold change compared to mean control. All
statistical analysis was calculated using GraphPad Prism 7 for Mac
(GraphPad Software, San Diego, California, USA; RRID:SCR_002798).
Statistical analysis was only undertaken for studies where each group
size was at least n = 4. For western blot analysis,
representative data are shown where group size is n = 3. When the
mean of two experimental groups were compared, a two‐tailed Studentst ‐test was performed. Normally distributed data without repeated
measurements were assessed by a one‐way ANOVA followed by Bonferroni
correction if the F value reached significance. In all cases aP < 0.05 was deemed significant.