ii) Ibrutinib pre-treatment 48 h ZIP time course
Male C57BL/6 mice (10-12 weeks old) were pre-treated with ibrutinib (10 mg·kg-1; p.o.) or vehicle (5% DMSO, 30% cyclodextrin) 1 hour prior to zymosan challenge (100 ug; i.p.). Peritoneal exudate cells were harvested in ice-cold PEC harvest buffer (PBS, 5 mM EDTA) 2, 4, 16 and 48 h after zymosan challenge.