Anti-Ara h ELISAs
The ELISA was used to quantify the relative amounts of allergens present
in the protein extracts of each condition. In the case of Ara h 2, raw
and roasted protein extracts showed similarly high levels of detection
at a protein concentration of 1 µg/mL coated on the plate. However, in
the case of the autoclaved extract, detection was reduced by
approximately 50% for the same concentration (Figure 3A).
Figure 3B shows similar detection levels of Ara h 8 for raw and roasted
peanut proteins at high extract concentrations coated on the plate (1
mg/mL). Interestingly, the autoclaved peanut extracts detected no Ara h
8, independent of coated extract concentration.