Anti-Ara h ELISAs
The ELISA was used to quantify the relative amounts of allergens present in the protein extracts of each condition. In the case of Ara h 2, raw and roasted protein extracts showed similarly high levels of detection at a protein concentration of 1 µg/mL coated on the plate. However, in the case of the autoclaved extract, detection was reduced by approximately 50% for the same concentration (Figure 3A).
Figure 3B shows similar detection levels of Ara h 8 for raw and roasted peanut proteins at high extract concentrations coated on the plate (1 mg/mL). Interestingly, the autoclaved peanut extracts detected no Ara h 8, independent of coated extract concentration.