1H Nuclear Magnetic Resonance (NMR)
Spectroscopy
Six whole peanuts of each condition were placed in 10 mL of double
distilled water and soaked at room temperature for 48 hours. Three
samples per condition of the resulting solutions (1 mL each) were
evaporated under vacuum at 45°C for 1.5 hours and the resulting residue
was reconstituted in 0.6 mL of double distilled water. The three samples
were combined together to give a total volume of 1.8 mL, 450 μL of which
was collected for analysis.
1H NMR spectra were run on a Bruker 400 MHz NMR
spectrometer for analysis using the water suppression pulse sequence,
zgpr (Bruker standard sequence; Bruker, MA, USA). Thirty-two scans were
taken with an acquisition time of 3 s and a spectral width of 12 kHz.
The 1H chemical shifts were internally referenced by
adding 0.5 mM of deuterated
3-(trimethylsilyl)propionic-2,2,3,3,-d4 acid sodium salt
(TSP-d 4; Sigma-Aldrich, ON, Canada) set to 0.0
ppm.