Acknowledgments
The authors are grateful to all who provided assistance during the preparation of this manuscript.
Declaration of interest : The authors declare that they have no competing interest.
Study Funding : This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Ethical committee: This study conforms the recognized standards according to the Declaration of Helsinki.
Author contributions :
Thomas Ntounis: Contributed to conception and design, drafted the manuscript, responsible for overall supervision.
Sofoklis Stavros: Contributed to conception and design, drafted the manuscript, responsible for overall supervision.
Antonios Koutras: Contributed to conception and design
Alexandros Katrachouras: Contributed to conception and design
Dimitrios Lentzaris: Contributed to conception and design
Aikaterini Domali: Responsible for overall supervision, drafted the manuscript
Kyriakos Konis: Responsible for overall supervision, drafted the manuscript
Peter Drakakis: Responsible for overall supervision, drafted the manuscript, overall revision.
All authors read and approved the final manuscript.
References
1. McAuley J. B. 2014. Congenital Toxoplasmosis. Journal of the Pediatric Infectious Diseases Society3 Suppl 1 (Suppl 1), S30–S35. https://doi.org/10.1093/jpids/piu077
2. Dubey, J. P., Miller, N. L., and Frenkel, J. K. 1970. The Toxoplasma gondii oocyst from cat feces. The Journal of experimental medicine132 (4), 636–662. https://doi.org/10.1084/jem.132.4.636
3. Montoya J. G. 2018. Systematic screening and treatment of toxoplasmosis during pregnancy: is the glass half full or half empty?. American journal of obstetrics and gynecology219 (4), 315–319. https://doi.org/10.1016/j.ajog.2018.08.001
4. Dunn, D., Wallon, M., Peyron, F., Petersen, E., Peckham, C., and Gilbert, R. 1999. Mother-to-child transmission of toxoplasmosis: risk estimates for clinical counselling. Lancet (London, England)353 (9167), 1829–1833. https://doi.org/10.1016/S0140-6736(98)08220-8
5. Daffos, F., Forestier, F., Capella-Pavlovsky, M., Thulliez, P., Aufrant, C., Valenti, D., and Cox, W. L. 1988. Prenatal management of 746 pregnancies at risk for congenital toxoplasmosis. The New England journal of medicine, 318(5), 271–275. https://doi.org/10.1056/NEJM198802043180502
6. Kusbeci, O. Y., Miman, O., Yaman, M., Aktepe, O. C., and Yazar, S. 2011. Could Toxoplasma gondii have any role in Alzheimer disease?. Alzheimer disease and associated disorders, 25(1), 1–3. https://doi.org/10.1097/WAD.0b013e3181f73bc2
7. Arias, I., Sorlozano, A., Villegas, E., de Dios Luna, J., McKenney, K., Cervilla, J., Gutierrez, B., and Gutierrez, J. 2012. Infectious agents associated with schizophrenia: a meta-analysis. Schizophrenia research136 (1-3), 128–136. https://doi.org/10.1016/j.schres.2011.10.026
Gay-Andrieu, F., Marty, P., Pialat, J., Sournies, G., Drier de Laforte, T. and Peyron, F. 2003. Fetal toxoplasmosis and negative amniocentesis: necessity of an ultrasound follow-up. Prenatal diagnosis23 (7), 558–560. https://doi.org/10.1002/pd.632
Swisher, C. N., Boyer, K. and McLeod, R. 1994. Congenital toxoplasmosis. The Toxoplasmosis Study Group. Seminars in pediatric neurology, 1(1), 4–25.
10. Kieffer, F. and Wallon, M. 2013. Congenital toxoplasmosis. Handbook of clinical neurology, 112, 1099–1101. https://doi.org/10.1016/B978-0-444-52910-7.00028-3
11. Olariu, T. R., Blackburn, B. G., Press, C., Talucod, J., Remington, J. S. and Montoya, J. G. 2019. Role of Toxoplasma IgA as Part of a Reference Panel for the Diagnosis of Acute Toxoplasmosis during Pregnancy. Journal of clinical microbiology, 57(2), e01357-18. https://doi.org/10.1128/JCM.01357-18
12. Rahbari, A. H., Keshavarz, H., Shojaee, S., Mohebali, M. and Rezaeian, M. 2012. IgG avidity ELISA test for diagnosis of acute toxoplasmosis in humans. The Korean journal of parasitology, 50(2), 99–102. https://doi.org/10.3347/kjp.2012.50.2.99
13.Sharifi, K., Hosseini Farash, B. R., Tara, F., Khaledi, A., Sharifi, K. and Shamsian, S. 2019. Diagnosis of Acute Toxoplasmosis by IgG and IgM Antibodies and IgG Avidity in Pregnant Women from Mashhad, Eastern Iran. Iranian journal of parasitology14 (4), 639–645.
14. Kodym, P., Machala, L., Rohácová, H., Sirocká, B. and Malý, M. 2007. Evaluation of a commercial IgE ELISA in comparison with IgA and IgM ELISAs, IgG avidity assay and complement fixation for the diagnosis of acute toxoplasmosis. Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases13 (1), 40–47. https://doi.org/10.1111/j.1469-0691.2006.01564.x
15. Olariu, T. R., Blackburn, B. G., Press, C., Talucod, J., Remington, J. S. and Montoya, J. G. 2019. Role of Toxoplasma  IgA as Part of a Reference Panel for the Diagnosis of Acute Toxoplasmosis during Pregnancy. Journal of clinical microbiology57 (2), e01357-18. https://doi.org/10.1128/JCM.01357-18
16.Kodym, P., Machala, L., Rohácová, H., Sirocká, B. and Malý, M. 2007. Evaluation of a commercial IgE ELISA in comparison with IgA and IgM ELISAs, IgG avidity assay and complement fixation for the diagnosis of acute toxoplasmosis. Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases13 (1), 40–47. https://doi.org/10.1111/j.1469-0691.2006.01564.x
17. Olariu, T. R., Blackburn, B. G., Press, C., Talucod, J., Remington, J. S. and Montoya, J. G. 2019. Role of Toxoplasma IgA as Part of a Reference Panel for the Diagnosis of Acute Toxoplasmosis during Pregnancy. Journal of clinical microbiology, 57(2), e01357-18. https://doi.org/10.1128/JCM.01357-18
18. Nascimento, F. S., Suzuki, L. A. and Rossi, C. L. 2008. Assessment of the value of detecting specific IgA antibodies for the diagnosis of a recently acquired primary Toxoplasma infection. Prenatal diagnosis, 28(8), 749–752. https://doi.org/10.1002/pd.2052
19. Breeze A. C. 2007. Infectious diseases of the fetus and newborn infant, 6th edn. Archives of Disease in Childhood. Fetal and Neonatal Edition92 (2), F156. https://doi.org/10.1136/adc.2006.102566
20. Paquet, C., Yudin, M. H. and Society of Obstetricians and Gynaecologists of Canada 2013. Toxoplasmosis in pregnancy: prevention, screening, and treatment. Journal of obstetrics and gynaecology Canada : JOGC = Journal d’obstetrique et gynecologie du Canada : JOGC35 (1), 78–81. https://doi.org/10.1016/s1701-2163(15)31053-7