Sample collection
2 ml venous blood from each rat’s eye socket was collected in the 1st,
3rd, 5th, and 6th courses of treatment and was kept in estrogen receptor
tubes at 4°C. When blood coagulation occurred, the tubes were placed in
a centrifuge. After centrifugation, the supernatant was collected and
kept in a freezer at -20°C. Rat vaginal smears were collected
simultaneously for H & E staining, which was done in Guangxi National
Hospital and observed by the microscope. All rats were sacrificed by
cervical dislocation under anesthesia.