2.6 | Proteases, protease inhibitors (PI) and
Excretory/secretory proteins (EPs)
Proteases and PIs were annotated using MEROPS batch-BLAST(Rawlings &
Morton, 2008) against MEROPS (E<1e-10). The
conventional secretion proteins and non-conventional secretion proteins
were identified. SignalP-5.0 Server
(http://www.cbs.dtu.dk/services/SignalP/) and TMHMM Server v2.0
(http://www.cbs.dtu.dk/services/TMHMM/) were used to predict the signal
peptides and transmembrane regions of proteins, respectively. The
proteins with NNscore > 0.9 were selected as
non-conventional protein secretion in the results of SecretomeP 2.0
Server
(http://www.cbs.dtu.dk/services/SecretomeP/)
identification.