Figure legends
Figure 1. Schematic representation of 2 × 3 factorial design.
Green-colored aphids indicate sham (non-infective) A. pisum ,
while blue-colored aphids indicate PEMV-infective A. pisum .
Slashes indicate order of S. lineatus treatments (S.
lineatus first, A. pisum first, or no S. lineatus ).
Figure 2. Relative transcript accumulation of plant hormone
biosynthesis genes associated with four hormonal signaling pathways: (A)ICS1 (salicylic acid), (B) LOX2 (jasmonic acid), (C)AOX3 (abscisic acid), and (D) GA2ox (gibberellic acid)
following attack with various combinations of S. lineatus ,A. pisum , and PEMV. Within each panel, bars separated by a
different letter were significant different based on MANOVA (Tukey HSD,
α = 0.05). Bar height and error bars indicate marginal mean and standard
error of the regression coefficient for each respective treatment.
Figure 3. Relative transcript accumulation of plant defense
response transcripts: (A) PR1, (B) DDR230, and (C)PsLectin following attack with various combinations of S.
lineatus , A. pisum , and PEMV. Within each panel, bars
separated by a different letter were significant different based on
MANOVA (Tukey HSD, α = 0.05). Bar height and error bars indicate
marginal mean and standard error of the regression coefficient for each
respective treatment.
Figure 4 . (A) Salicylic acid, (B) jasmonic acid, and (C)
abscisic acid phytohormone levels in P. sativum plants following
attack with various combinations of S. lineatus , A. pisum ,
and PEMV. Within each panel, bars not connected by the same letter are
significantly different (Tukey HSD, α = 0.05). Bar height and error bars
indicate marginal mean and standard error of the regression coefficient
for each respective treatment.
Figure 5 . Nutritional analysis (total amino acid) in P.
sativum following attack with various combinations of S.
lineatus , A. pisum , and PEMV. S. lineatus increase
total amino acid concentration in plants (GLM, χ2 = 9.194, P =0.01). There was no “sham-none” treatment combination so that could
not be estimated. Within each panel, bars not connected by the same
letter are significantly different (Tukey HSD, α = 0.05). Bar height and
error bars indicate marginal mean and standard error of the regression
coefficient for each respective treatment.