Material methods
This study was approved by Our University Animal Experiments Local
Ethics Committee [26.04.2017, Protocol No: 2017/011]. Twenty eight
female wistar albino rats weighing 160-250 g were included in the study.
Experimental animals were kept in our University Animal Laboratory at a
room temperature of 24 ° C and a 12/12 hour day and night cycle. Fed
with standard rat food and water without restrictions. Each rat was
anesthetized with ketamine hydrochloride [40 mg/kg iv]. Before
surgery, The abdominal area of the rats was shaved and wiped with 1%
povidone iodine and prepared for the operation, approximately 4 cm lower
midline laparotomy was performed on the umbilical region. Before
surgery, the rats were randomly divided into four groups each consisting
of 7 rats. The operations performed in groups of rats were as follows:
- Control group [CG]; a standard adhesion was created, no adjuvant
was given
- Corn oil group [CoG]; After the injury, 1 ml of corn oil was
administered intraperitoneally. The same dose was continued
intraperitoneally for 14 days.
- Low-dose lycopene group [LLG]; After the injury, 5 mg/kg lycopene
was administered intraperitoneally. The same dose was continued
intraperitoneally for 14 days.
- High-dose lycopene group [HLG]; After injury, only a single dose
of 20 mg / kg lycopene was administered intraperitoneally.
The dose of lycopene was chosen based on other studies
(11-13)
After a 4 cm abdominal incision, the uterine horns were exposed and a
lesion was created on the antimesenteric surface of each uterine horn
surface with the help of 10 wolt bipolar cautery. At the same time,
extra adhesion was created by scraping operations on the visceral
surface until serosal bleeding occurred. After the procedure was
completed, the abdomen was closed continuously using 4.0 vicryl. The
rats were then allowed to recover for two weeks. Two animals in the LLG
and two animals in the HLG died after the first surgical procedure, so
these groups were evaluated out of 5 animals. After the recovery period,
the animals were sacrificed and evaluated for adhesion formations. The
researchers who evaluated the adhesions had no prior knowledge of which
group the rat belonged to. Adhesion scoring based on extend, severity,
and resistance to applied force is summarized in table 1. The sum of the
three parameters was used as the total score for each group.
Tissue samples taken from the peritoneum and adhesion area were sent for
histopathological examination. Adhesion examples are shown in figure 1.