Evaluation of immunohistochemistry staining
Immuno stained preparations were evaluated under light microscopy [Nicon Eclips 80i, Japan]. The randomly determined areas in the preparations were examined by the researcher blindly [without reporting the groups]. The staining intensity was scored as no [-] [0], light [1] [+], medium [2] [++], strong [3] [+++] and thus the number of reactive cells was determined. After scoring, photos were taken from the sections with the image analysis system. H-Score values were calculated after counting positive stained cells in each 100 cells in 3 randomly selected fields for each group [H-Score: ∑Pi [i + 1] [Pi:% number of positively stained cells; i: staining intensity].