Statistical analysis
SPPS 25 [IBM Corp. Released 2017. IBM SPSS Statistics for Windows,
Version 25.0. Armonk, NY: IBM Corp.] statistical package program was
used to evaluate the data. Histopathological changes between
experimental groups were analyzed comparatively. Experiments were done
independently in 6 replicates. Variables are expressed using mean ±
standard deviation, percentage and frequency values. Variables were
evaluated after checking the preconditions for normality and homogeneity
of variances [Shapiro Wilk and Levene Test]. For comparison of three
or more groups, the one-way anova test and the Tukey HSD test, one of
the multiple comparison tests, were used. Categorical data were analyzed
with Fisher’s Exact Test and Chi-Square test. In cases where the
expected frequencies are lower than 20%, an evaluation was made with
the ”Monte Carlo Simulation Method” to include these frequencies in the
analysis. For the significance level of the tests, p <0.05 and
p <0.01 values were accepted.