Measurement of total antioxidant capacity [TAC]
Measurement of total antioxidant capacity in tissue samples was
performed spectrophotometrically with a commercial kit [Rel Assay
Diagnostics]. According to the principle of this method, the
antioxidant molecules in the studied sample cause the formation of a
lighter colored compound by reducing the dark blue-green colored ABTS
radical. The absorbance of the resulting color is read in the
spectrophotometer at a wavelength of 660 nm
(18). The level of antioxidant molecules
in the sample is calibrated with the stable antioxidant compound Trolox
Equivalent [a vitamin E analog] present in the kit.