Hematoxylin-Eosin [H-E] staining method
With this method, it is aimed to determine the morphological properties
of the preparations at the light microscope level. After the
preparations were deparaffinized, they were rehydrated with decreasing
concentrations of ethanol [99.6%, 96%, 90%, 80%, 70%, 50%].
Tissues held for 2 minutes on each alcohol batch. After staining with
hematoxylin for 3 minutes, it was soaked in tap water for 5 minutes and
removed by immersion in ammonia water. Then the preparations were
stained with eosin for 3 minutes and washed with distilled water for 5
minutes. Tissues were kept in pure xylene solution for 1 minute after
waiting for 2 minutes in increasing concentrations of ethanol series
[50%, 70%, 80%, 90%, 96%, 99.6%]. Sections closed with
enthallen were examined with Nikon Eclipse 80i image analysis system
(15).