Evaluation of immunohistochemistry staining
Immuno stained preparations were evaluated under light microscopy
[Nicon Eclips 80i, Japan]. The randomly determined areas in the
preparations were examined by the researcher blindly [without
reporting the groups]. The staining intensity was scored as no [-]
[0], light [1] [+], medium [2] [++], strong [3]
[+++] and thus the number of reactive cells was determined. After
scoring, photos were taken from the sections with the image analysis
system. H-Score values were calculated after counting positive stained
cells in each 100 cells in 3 randomly selected fields for each group
[H-Score: ∑Pi [i + 1] [Pi:% number of positively stained cells;
i: staining intensity].