Sample Collection
Subjects inhaled 2×100 µg salbutamol (nominal dose 200 µg). Salbutamol
was delivered via their own MDI device. The inhaler delivered a nominal
dose of 100 µg per actuation. Subjects emptied their bladders
immediately before drug administration and then urine samples were
collected 30 min after the inhaled doses. The collected urine samples
were recorded and stored at −20 °C before analysis. A previously
validated, sensitive high-performance liquid chromatography (HPLC)
method was used to measure urine concentrations of
salbutamol.16 Subjects did not drink alcohol or
caffeine-containing beverages for 12 h before or 24 h after treatment.
Demographic details like age, sex, disease characteristics were
collected from each recruited patient. The study was approved by the
Institutional Ethics Committee of the University (525/IHEC/3-19).