2.11 Sucrose Preference Test (SPT)
This test lasted for 4 days and was carried out according to previously reported methods with some modifications(Li-Wen et al., 2020). On the first 2 days, the C57BL/6J mice were free to drink two bottles containing pure water and 1% sucrose solution, respectively. And the position of two bottles was changed every 6 h to prevent potential location preference of drinking. On the 3rd day, both the food and two bottles were deprived for 24 hours. On the 4th days, the test lasted for 6 hours, with the position of the bottles interchanged and the two bottles weighed before and after the test period. Sucrose preference was calculated as the percentage of sucrose solution ingested relative to the total amount of liquid intake. Drug treatments were not given during the testing days.