2.14 Statistical analysis
All analyses were performed using SPSS 24.0 software (SPSS Inc., USA),
and data are presented as mean ± SEM (standard error of the mean). The
comparisons between groups were evaluated using one-way ANOVA (post hoc
LSD test) or two-way ANOVA (post hoc Bonferroni’s test), as appropriate.
For all one-way ANOVAs, post hoc tests were performed using LSD test
(the least significant difference test). For all two-way ANOVAs,
Bonferroni post hoc tests were used to assess isolated comparisons. A
value of P < 0.01 was considered statistically significant.
The data and statistical analysis
comply with the recommendations on experimental design and analysis in
pharmacology.