2.14 Statistical analysis
All analyses were performed using SPSS 24.0 software (SPSS Inc., USA), and data are presented as mean ± SEM (standard error of the mean). The comparisons between groups were evaluated using one-way ANOVA (post hoc LSD test) or two-way ANOVA (post hoc Bonferroni’s test), as appropriate. For all one-way ANOVAs, post hoc tests were performed using LSD test (the least significant difference test). For all two-way ANOVAs, Bonferroni post hoc tests were used to assess isolated comparisons. A value of P < 0.01 was considered statistically significant. The data and statistical analysis comply with the recommendations on experimental design and analysis in pharmacology.