Microinjection of drugs into the insular cortex
Injection needles (12 mm long, 33 G, Small Parts, USA) were connected to a 1 µl syringe (Hamilton, USA) through a polyethylene tube (PE-10) for the microinjection of drugs into the brain. The final volume of microinjection was 100 nL/side (Alves, Crestani, Resstel, & Correa, 2014; Alves et al., 2013).