Effects of microinjection of LY235959 or NBQX into the insular
cortex on baroreflex activity during restraint stress
aCSF - Bilateral microinjection of artificial cerebrospinal
fluid (aCSF, 100 nL) into the IC did not change the basal values (before
stress) of the baroreflex effectiveness index (Table 2) or the
baroreflex gain (Table 3).
LY235959 – Bilateral microinjection of LY235959 did not
change the basal values (before stress) of the baroreflex effectiveness
index (Table 2) or the baroreflex gain (Table 3). Spontaneous baroreflex
analysis demonstrated that microinjection of the selective NMDA
glutamate receptor antagonist LY235959 (1 nmol/100 nL) into the IC
decreased the BEI for the UP (F(1,10) = 10.09), ALL
(F(1.10) = 11.46) and DOWN sequences
(F(1,10) = 7.85) (Fig. 5). The baroreflex gains of the
UP, DOWN and ALL sequences were not altered by IC treatment with
LY235959 (UP: F(1,10) = 1.984; DOWN:
F(1,10) = 1.245; and ALL: F(1,10) =
4.689) (Fig. 5).
NBQX – Bilateral microinjection of NBQX did not change
the basal values (before stress) of the baroreflex effectiveness index
(Table 2) or the baroreflex gain (Table 3). Bilateral
microinjection of the selective non-NMDA antagonist NBQX (1 nmol/100 nL)
into the IC did not change the BEI in any of the sequences (UP:
(F(1,8) = 0.8254; DOWN: F(1,8) =
0.07898; and ALL: F(1,8) = 0.3413). The baroreflex gains
for the UP, DOWN and ALL sequences were also not changed by IC treatment
with NBQX (UP: F(1,8) = 0.9174; DOWN:
F(1,8) = 1.305; and ALL: F(1,8) =
0.4202) (Fig. 5).