Effects of microinjection of LY235959 or NBQX into the insular cortex on baroreflex activity during restraint stress
aCSF - Bilateral microinjection of artificial cerebrospinal fluid (aCSF, 100 nL) into the IC did not change the basal values (before stress) of the baroreflex effectiveness index (Table 2) or the baroreflex gain (Table 3).
LY235959 Bilateral microinjection of LY235959 did not change the basal values (before stress) of the baroreflex effectiveness index (Table 2) or the baroreflex gain (Table 3). Spontaneous baroreflex analysis demonstrated that microinjection of the selective NMDA glutamate receptor antagonist LY235959 (1 nmol/100 nL) into the IC decreased the BEI for the UP (F(1,10) = 10.09), ALL (F(1.10) = 11.46) and DOWN sequences (F(1,10) = 7.85) (Fig. 5). The baroreflex gains of the UP, DOWN and ALL sequences were not altered by IC treatment with LY235959 (UP: F(1,10) = 1.984; DOWN: F(1,10) = 1.245; and ALL: F(1,10) = 4.689) (Fig. 5).
NBQX Bilateral microinjection of NBQX did not change the basal values (before stress) of the baroreflex effectiveness index (Table 2) or the baroreflex gain (Table 3). Bilateral microinjection of the selective non-NMDA antagonist NBQX (1 nmol/100 nL) into the IC did not change the BEI in any of the sequences (UP: (F(1,8) = 0.8254; DOWN: F(1,8) = 0.07898; and ALL: F(1,8) = 0.3413). The baroreflex gains for the UP, DOWN and ALL sequences were also not changed by IC treatment with NBQX (UP: F(1,8) = 0.9174; DOWN: F(1,8) = 1.305; and ALL: F(1,8) = 0.4202) (Fig. 5).