Data and Statistical analysis
All analysis were performed using Prism 6.0 software (GraphPad, USA) and followed the guidelines on experimental design and analysis in pharmacology (Curtis et al., 2018). Data are expressed as the mean ± standard error of the mean (SEM). The paired Student’s t test was used to compare basal values of MAP, HR, tail skin temperature and spontaneous baroreflex analysis before and after drug treatment. We used the unpaired Student’s t test to compare baseline corticosterone values (before stress) between the control and drug treatments. Changes in cardiovascular parameters, tail skin temperature, spontaneous baroreflex and corticosterone levels evoked by restraint stress were analysed using two-way ANOVA with treatment as the main factor (vehicle x drugs) and time as repeated measures followed by Bonferroni’spost hoc test. P<0.05 was set as significant.