3.3 Immune response to vaccination of recombinant TM-1
To evaluate the humoral immune response to vaccination of recombinant TM-1, serum from vaccinated chickens were analyzed with ELISA against anti-MG antibody weekly starting from the first week after the initial vaccination. The titer of anti-MG antibody started to increase in TM-1 and MG attenuated vaccine groups one week after initial immunization, although not significantly higher compared to control groups, until two weeks later (Fig. 4). Moreover, while MG attenuated vaccine caused higher anti-MG antibody titer, it is not significantly different from recombinant TM-1 group (Fig. 4). Notably, amount of anti-MG antibody was improved even more one week after second vaccination, leading to a greater significance compared to control group vaccinated with WT and PBS buffer (Fig. 4).