3.3 Immune response to vaccination of recombinant TM-1
To evaluate the humoral immune response to vaccination of recombinant
TM-1, serum from vaccinated chickens were analyzed with ELISA against
anti-MG antibody weekly starting from the first week after the initial
vaccination. The titer of anti-MG antibody started to increase in TM-1
and MG attenuated vaccine groups one week after initial immunization,
although not significantly higher compared to control groups, until two
weeks later (Fig. 4). Moreover, while MG attenuated vaccine caused
higher anti-MG antibody titer, it is not significantly different from
recombinant TM-1 group (Fig. 4). Notably, amount of anti-MG antibody was
improved even more one week after second vaccination, leading to a
greater significance compared to control group vaccinated with WT and
PBS buffer (Fig. 4).