Figure 4. Immune responses to recombinant TM-1 vaccine . Anti-MG antibodies in chicken serum were quantified by ELISA after oral delivery of recombinant TM-1 vaccine, WT, and PBS except for group innoculated with attanuated MG vaccine [MG(av)] by injection. (V) and (C) represent the week when vaccination or challenge occured respectively. *, P <0.05, **, P <0.01, ***,P <0.001.