Fig. 2. Process of developing transgenic plant using Biolistics: Segregation of transgenic wheat lines : A1. Immature embryos after bombardment with plasmid carying TM-1. A2. Callus development under dark with antibiotic selection. A3. Plant regeneration in media containing selection marker. A4. Plant regeneration and rooting in growth chamber. A5. Wheat plant expressing TM-1 vaccine candidate in rooting media, before moving to soil for growth. B. Embryo rescue analysis. Wheat embryo excised from WT and positive plants and their germination on selection media. B. Embryo excision for transgene selection with kanamycine supplemented germinating media. B1. Wild type BobWhite as negative control. B2. Growth TM-1 positive embryo excised from seeds after 10 days on MS half media as control for the experiment.