3.2 Changes in the protein expression of Ang1, Ang2, Twist1 and
Tie2 in primary HUVECs under hyperoxia
Previously, we used western blot to detect the expression of Ang1, Ang2,
Twist1 and Tie2 proteins in the lung tissue of hyperoxia neonatal
rats20, but did not further verify the changes of
these proteins by performing in vitro experiment. As shown in
Figure 2, compared with the control group, the protein expression of
Twist1 (p <0.05) (Figure 2A, 2B), Tie2 (p <0.01)
(Figure 2A,2C) and Ang1 (p <0.01) (Figure 2A,2D) were
significantly reduced in the hyperoxia group. In contrast, Ang2 protein
expression in the hyperoxia group was significantly higher than that in
the control group (p <0.01) (Figure 2A, 2E). The results of
this in vitro experiment were consistent with those derived from
the in vivo experiment.