2.8 Data analysis
Experimental data were processed by GraphPad Prism version 8.0 software
and Image J software. Statistical analysis was carried out with SPSS
version 24.0. Measurement data are expressed by mean ± standard
deviation. Comparisons between the two groups (that were normally
distributed and showed homogenous variances) were carried out with the
independent two-sample t-test. Comparisons between multiple groups were
performed by one-way analysis of variance (ANOVA). The rank sum test was
used when the normal distribution and variance homogeneity test were not
satisfied; p< 0.05 was considered to be statistically
significant.