2.8 Data analysis
Experimental data were processed by GraphPad Prism version 8.0 software and Image J software. Statistical analysis was carried out with SPSS version 24.0. Measurement data are expressed by mean ± standard deviation. Comparisons between the two groups (that were normally distributed and showed homogenous variances) were carried out with the independent two-sample t-test. Comparisons between multiple groups were performed by one-way analysis of variance (ANOVA). The rank sum test was used when the normal distribution and variance homogeneity test were not satisfied; p< 0.05 was considered to be statistically significant.