Reactive Oxygen Species (ROS)
In order to investigate whether E2 modulates ROS production post-LPS, we stratified the samples according to sex and performed flow cytometry. LPS non-significantly increased ROS production in neonates and adults and this effect is not sex-specific (Figure 4). Female neonates had significantly less ROS production following E2 and LPS treatment in combination compared to male neonates (p<0.05) (Figure 4).