Fatty acid composition
To prepare FAME, 0.3 g of the oil samples was dissolved in 7 ml of
hexane, followed by vigorous shacking of the solution with 2 ml of 7 N
methanolic potassium hydroxide (KOH) at 50 °C for 10 min. FAME were
identified using an HP-5890 gas chromatograph (Hewlett-Packard, CA, USA)
equipped with a CP-FIL 88 (Supelco, Inc., Bellefonte, PA) capillary
column of fused silica (60 m × 0.22 mm I.D., 0.2 µm film) and a flame
ionization detector (FID). Nitrogen was used as carrier gas with a flow
rate of 0.75 ml min–1. The oven temperature was
maintained at 198 °C, and those of injector and detector at 250 °C.