2.1 Genome sequence retrieval
The CoV-2 genome sequences were retrieved from global science initiative
on sharing all influenza data (GISAID) (Dec, 2019-Dec, 2020)
(https://www.gisaid.org/) [12]. About 295000 genomes of CoV-2
reported worldwide, were screened for variants in structural proteins.
All the sequences were aligned with reference genome of Wuhan-Hu-1
(Accession NC_045512) using CoVsurver application
(https://www.gisaid.org/epiflu-applications/covsurver-mutations-app/).
All the identified mutations in structural proteins of CoV-2 were
separated and arranged in the form of excels sheets. The statistical
analysis was performed to screen the most common variants. The GISAID is
sharing the virus data to publish results and the metadata that concerns
of public health scientists. The GISAID is an effective server for rapid
sharing all kind of data including unpublished influenza data.