Figure2. Spotting test
5 out of 12 yeast samples in complex backgrounds or not were taken and
diluted to 2×107 cells per ml, and then the diluent
samples were subjected to 10-fold dilutions. 5 μl of each dilution were
spotted onto YPD solid media. Thus, the numbers at the top of images
indicate the CFUs in theory. (A) Manual counting results for samples of
simple backgrounds. (B) Automatic counting results for samples of simple
backgrounds with “Yeast Counter”. (C) Manual counting results for
samples of complex backgrounds. (D) Automatic counting results with
ilastik and ImageJ for samples under complex backgrounds.