Bone marrow chimeras
Recipient
mice were 2–3-month-old B6 mice (CD45.1). Mice were irradiated with 7
Gy X-ray and then immediately injected intravenously (i.v.) with 5 ×
106 BM cells from WT or CCR2 KO (CD45.2) under aseptic
condition. Cells from WT or CCR2 KO mice were mixed with that of CD45.1
mice at a 1:1 ratio. One week before the radiation and 2 weeks after the
injection, mice were given water with antibiotics (gentamicin and
erythromycin). Eight weeks after the transfer and the sacrifice of the
mice, FCM was performed.