Sex-specific sequence polymorphism in Macquarie perch, but not its
three relatives, revealed by amplicon sequencing
For Macquarie perch, genotypes for the ~400-bp region of
scaffold 633 captured by amplicon sequencing using F2-R3 primers were
consistent with WGS genotypes for the two females and two males from
Dartmouth (Appendix G, Table G1). Amplicon sequences for the two
individuals from coastal populations (Table G1) showed that only
variation at 93229 (the site chosen for the PCR-RFLP sexing assay) might
be consistently sex-linked across sequenced individuals. Unique
variation at the 146-bp sexing region in two coastal individuals of
unknown sex further suggests that sex-linked variation in Macquarie
perch could be population-specific. Genotypes for the amplicon-sequenced
fragment homologous to the Macquarie perch sex-linked region did not
reveal clear sex-linked variation in golden perch (Table G2), Murray cod
or trout cod (Table G3).