Drugs and choice of doses
Molecules were purchased from Abcam Biochemicals (Abcam Biochemicals,
Cambridge, United Kingdom), Biotrend (BioTREND, Chemikalien GmbH, Koln,
Germany), Carbosynth (Carbosynth Ltd, Berkshire, United Kingdom),
Clinisciences (CliniSciences, Nanterre, France), Sequoia Research
Product ltd (Sequoia Research Products, Pangbourne, United Kingdom) and
Sigma-Aldrich (Saint Quentin, France). The list of pharmacological
agents used for validation of the pulse contour model is provided in
Supplemental Material 1, Table 1. The full list of tested drugs
including suppliers, vehicles and volumes of administration depending on
dose levels and route administration is provided in Supplemental
Material 2, Table 2. Doses were selected from preliminary trials to
determine the minimum effective dose causing either QTc prolongation,
increase in HFQT oscillations or change in autonomic control. A set of
experiments were also conducted at higher doses with dofetilide,
moxifloxacin, quinidine, sotalol and thioridazine.