Statistical procedures
Comparisons between groups were performed using an analysis of variance for repeated measures and Dunnett’s test. The level of probability (P) deemed to constitute the threshold for statistical significance was fixed to P≤0.05. Results are expressed as mean values ± SEM. The exact group size (n) is provided in legends of tables and figures. “n” values refer to independent animals or subjects and not replicates. Statistics were performed using RS/1 release 6.3, Applied Materials. Operators for animal experiments and data analysts were not blinded. This work does not contain any subjective observation or analyses that would justify blinding. Furthermore, all calculations were made by using fully automated computer procedures (GLP validated) from beat to beat analysis up to statistics.