Conclusion
This study showed that the SRB method in the diagnosis of HD provides a great advantage over full-thickness rectal biopsy because it could be performed safely and without anaesthesia due to the very low rate of serious complications in both newborn period and older children. In addition, when insufficient material was obtained in HD diagnosis neglected, considering that the sensitivity and specificity rates were very high, could be applied quickly and easily for the diagnosis of HD. And also, it provides an advantage when performing definitive operations after possible HD, rectal biopsy, which is the gold standard method for the diagnosis of HD, is primarily we consider that it should be obtained with the rectal aspiration biopsy method.