ABSTRACT
Purpose: In this prospective cross secitonal study, we aimed to
investigate the adequacy, sensivity and specifity of rectal aspiration
biopsy samples as the standart diagnosis of patients suspected HD for
the histopathological diagnosis.
Material and methods: The study was performed between november
2016 and march 2018. We prospectively included 24 patients aged between
0-3 years that suspicious with HD to the study. After Rectosigmoid index
(RSI) were calculated according to the contrast enhanced colon graphy,
Patients who RSI<1 were performed rectal aspiration biopsy
with the suspicious of HD. All patients’ clinical features, treatment
options, complications, laboratory results and radiological findings
were recorded for further analyses. Biopsy specimens were evaluated by
the specialist patholog and findings were recorded.
Results: There were no ganglion on the specimens of rectal
aspiration biopsy in 10(%41.6) patients and diagnosed as HD. Ganglion
cell was detected in 5(%20.8) patients and diagnosis of HD was
excluded. Inadequate or suspicious biopsy specimens for
histopathological evaluation was observed in 9(%37.5) patients. If the
biopsy volume was greater than 4 mm3, sensitivity was 80% and
specificity was 66.67% in diagnosis of HH (AUC = 0.789); If the
Submucosa / Mucosa ratio is greater than 0.75 or the submucosa is
greater than 0.42, the sensitivity and the specificity were 86.67% and
66.67%, respectively.
Conclusion: In this prospective cross-sectional study, we
demonstrated that rectal aspiration biopsy in the diagnosis of HD is
easily feasible, safe, has higher sensitivity and specificity, and lower
complication rates.
Keywords: Hirschsprung Disease, Ganglion, Rectal Aspiration
Biopsy, Rectal Biopsy