Reagents, Equipment, and Kits
Chloroauric acid was purchased from Sigma-Aldrich (St Louis, MO, USA);
tri-sodium citrate was purchased from the Sinopharm Group (Shanghai,
China); sample pads, absorbent pads, conjugate pads, nitrocellulose (NC)
membranes, and PVC sheets were purchased from Shanghai Jiening Biotech
Co., Ltd. (Shanghai, China); potassium carbonate was purchased from
Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China); triple distilled
water was used for preparing colloidal gold experiments. The ultrapure
water system was purchased from MilliporeSigma (Burlington, MA, USA); an
electronic balance model JY2001 was purchased from Shanghai Precision
Science Instrument Co., Ltd. (Shanghai, China); the Biodot AD3200 gold
standard diagnostic spotting system and Biodot CM4000 flat top fully
automated stripper were obtained from BioDot Inc., (Irvine, CA, USA); an
African swine fever (p30) ELISA antibody detection kit (lot number:
B22013) was purchased from Svanova (Uppsala, Sweden); an African swine
fever (p72) ELISA antibody assay kit (lot number: 171218) was purchased
from Ingenasa (Madrid, Spain).