Reagents, Equipment, and Kits
Chloroauric acid was purchased from Sigma-Aldrich (St Louis, MO, USA); tri-sodium citrate was purchased from the Sinopharm Group (Shanghai, China); sample pads, absorbent pads, conjugate pads, nitrocellulose (NC) membranes, and PVC sheets were purchased from Shanghai Jiening Biotech Co., Ltd. (Shanghai, China); potassium carbonate was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China); triple distilled water was used for preparing colloidal gold experiments. The ultrapure water system was purchased from MilliporeSigma (Burlington, MA, USA); an electronic balance model JY2001 was purchased from Shanghai Precision Science Instrument Co., Ltd. (Shanghai, China); the Biodot AD3200 gold standard diagnostic spotting system and Biodot CM4000 flat top fully automated stripper were obtained from BioDot Inc., (Irvine, CA, USA); an African swine fever (p30) ELISA antibody detection kit (lot number: B22013) was purchased from Svanova (Uppsala, Sweden); an African swine fever (p72) ELISA antibody assay kit (lot number: 171218) was purchased from Ingenasa (Madrid, Spain).