+ + +, the effective rate was 100%; + +, the effective rate was 66%; +, the effective rate was 33%; －, the effective rate was 0%.

Pig NO.1 p30 and p72 antibodies were detected gradually from the 7th and 9th day, respectively, and then at a steady upward trend (Figure 10A ); Pig NO.2 p30 and p72 antibodies were detected gradually from the 7th and 9th day, respectively, and then at a steady upward trend (Figure 10B ); Pig NO.3 p30 and p72 antibodies were gradually detected from the 9th and 11th day, respectively, and then at a steady upward trend (Figure 10C ); Both p30 and p72 antibodies of pig NO.4 were detected gradually from day 11, and then at a steady upward trend (Figure 10D ); Pig NO.5 p30 and p72 antibodies were gradually detected from the 11th and 15th day, respectively; afterward, a steady upward trend was observed (Figure 10E ). As shown inFigure 10F and Figure 10G , the above results are consistent with the test results of the commercial ELISA test kits (the Svanova p30 indirect ELISA result was positive when the PP value was greater than 10, and the Ingenasa p72 blocking ELISA was positive when the blocking rate was greater than 40%).

FIGURE 10│ Five pigs were immunized with the attenuated Δ181/UK strain; the test strips prepared in this study, the Svanova p30 and Ingenasa p72 ELISA kits, were used to detect antibody growth and decline. A–E: Pigs No. 1–5 test strip antibody growth and decline results; F: Pigs No. 1–5 Svanova p30 ELISA results; G: Pigs No. 1–5 Ingenasa p72 ELISA results.