ICS Result and Reaction Principle
The serum was diluted with the sample diluent at a ratio of 1:10, and
then 100 μL of the diluted sample solution was dropped onto the sample
pad to observe the results within 5–10 min at room temperature. If the
test and control lines appeared red,
the test was positive; if the test
line did not appear red, and the control line appeared red, the test was
negative. If the control line was not colored, the test was deemed
invalid (Figure 7B ).
When the serum to be tested is added to the sample pad, it migrates
forward by capillary action. If the serum reaching the conjugate pad
contains p30 or p72 antibodies, the protein on the conjugate pad reacts
with its corresponding antibody, thereby forming the corresponding
antigen-antibody complex. It continues to migrate to T2 (p30) or T1
(p72) to form a red band visible to the naked eye. Regardless of whether
the serum to be tested contains p30 and p72 antibodies, the gold-labeled
protein will continue to migrate to the control line and react with the
anti-p30 and -p72 monoclonal antibody complexes at the control line to
produce a red band visible to the naked eye. Therefore, if the red bands
are not displayed during the quality control assessment, the test strip
is rendered invalid.