Identification and Purification of p72, p30 Recombinant Protein
The expressed p30 recombinant protein and p72 truncated protein were purified by nickel column chromatography and subjected to SDS-PAGE, resulting in bands of approximately 45 and 38 kDa, respectively. This indicated that the purified protein of interest had a single band that was consistent with the predicted band size, and the target protein was purified successfully (Figure 1 ).
Figure 1│ Purification of p30 recombinant protein and p72 truncated protein. 1: SDS-PAGE analysis of p30 recombinant protein, 2: SDS-PAGE analysis of p72 truncated protein, M: Marker.