Validation of the LSS
The accuracy of the predicted AUC0–∞ by one-point and
two-point sampling strategies was determined in a cohort of seven
patients who were not included in the development of the LSS (Table 4).
All seven patients weighed ≤23 kg. By the one-point sampling strategy,
the predicted AUC0–∞ had more than 15% errors compared
to the actual AUC0–∞ in four patients. In contrast,
none of the patients had more than 15% error by the two-point sampling
strategy.