Validation of the LSS
The accuracy of the predicted AUC0–∞ by one-point and two-point sampling strategies was determined in a cohort of seven patients who were not included in the development of the LSS (Table 4). All seven patients weighed ≤23 kg. By the one-point sampling strategy, the predicted AUC0–∞ had more than 15% errors compared to the actual AUC0–∞ in four patients. In contrast, none of the patients had more than 15% error by the two-point sampling strategy.