Figure 7. BW-031 inhibits cough in guinea pigs following allergic airway inflammation.
a , Experimental design. Guinea pigs were sensitized with intraperitoneal and subcutaneous ovalbumin and challenged with inhaled (inh.) ovalbumin two weeks later. One day after ovalbumin challenge, the animals inhaled BW-031 (inhaled doses of 1.82, 7.07 or 17.6 mg/kg BW-031) followed one hour later by inhalation of 400 mM citric acid (yellow) for 7 minutes. Cough counts were measured for 17 minutes during and following the citric acid challenge. b , Ovalbumin sensitization and challenge causes lung inflammation, as measured by immune cell counts in bronchoalveolar lavage (BAL). N=12-20 animals per group (1:1 male:female), 1-way ANOVAs; total immune cell [F(1, 30) =10.3], p=0.003; neutrophils [F(1, 30)=5.82], p=0.02; eosinophils [F(1, 30)=11.9], p=0.002; monocytes [F(1, 30)=7.00], p=0.01; lymphocytes [F(1, 30)=10.9], p=0.003; Tukey’s post-hoc, *p<0.05, **p<0.01. Bars represent mean±SEM for each condition, while the individual data points are displayed as open circles. c , Inhaled BW-031 produces dose-dependent inhibition of citric acid-evoked cough following allergic airway inflammation. N=12 animals per group (1:1 male:female), 1-way ANOVA, [F(3, 44)=7.113], p=0.0005; Tukey’s post-hoc, **p<0.01, ***p<0.001. Bars represent mean±SEM for each condition, while the individual data points are displayed as open circles.