Figure 5. BW-031 produces long-lasting analgesia in a mouse paw UV-burn model and has no effect on sensory or motor function with perisciatic injection in naïve mice.
a , Von Frey measurements of hindpaw mechanical sensitivity in mice after plantar UV-burn and intraplantar injection of vehicle, 2% QX-314 or 2% BW-031. Two-way repeated measures ANOVA with treatment as the between groups factor and time as the within groups factor. Treatment [F(2, 27)=291.1], time [F(3.129, 84.49)=44.83] and treatment x time interaction [F(10, 135)­­=37.80], all p<0.001. Post-hoc Tukey’s tests between treatment groups at each time point revealed significant increases in mechanical threshold by QX-314 and BW-031 at 1, 3, 5 and 7 hours post treatment as compared with vehicle, with BW-031 treatment producing a larger mechanical threshold than QX-314 at 3, 5 and 7 hours after treatment. N=10 male mice per group, *p<0.05, **p<0.01, ***p<0.001. b , Toe spread assay of motor function in mice after perisciatic injection of 0.5% lidocaine, 0.5% QX-314 or 0.5% BW-031. Only lidocaine produces robust block of motor function in naïve mice. N=10 male mice per group, 1-way ANOVA (5 min time point), [F(2, 21)=81], p=1.3x10-10; Tukey’s post-hoc, ***p<0.001. Data are mean±SEM. c , Plantar pinprick responses in naïve mice after perisciatic injection of 0.5% lidocaine, 0.5% QX-314 or 0.5% BW-031. Only lidocaine produces robust sensory analgesia in naïve mice. N=10 male mice per group, Fisher’s exact test (5 min time point), p=4.1x10-6, ***p<0.001. Data are mean±SEM.