Figure 4. BW-031 produces long-lasting analgesia in rat paw CFA
injection and surgical paw incision pain models.
a, Hargreaves assay of hindpaw thermal sensitivity in rats
after intraplantar injection of Complete Freund’s Adjuvant (CFA) alone
(white), 2% QX-314 dissolved in CFA (blue) or 2% BW-031 dissolved in
CFA (red). Both QX-314 and BW-031 produce robust thermal analgesia.
Two-way repeated measures ANOVA with treatment as the between groups
factor and time as the within groups factor. Treatment [F(2,
27)=15.05], time [F(1.980, 53.46)=14.88], and treatment x time
interaction [F(4, 54)=6.767], all p<0.001. Post-hoc
Tukey’s tests between treatment groups at each time point revealed
significant increases in mechanical threshold by BW-031 at 1 and 4 hours
post treatment and QX-314 at 1h post treatment. N=10 male rats per
group, *p<0.05, **p<0.01, ***p<0.001.b , Von Frey measurements of hindpaw mechanical sensitivity in
rats after paw incision and intraplantar injection of vehicle or 2%
BW-031. Baseline measurements were made before paw incision. Vehicle or
2% BW-031 was injected 24 hours after the incision. Two-way ANOVA
(mixed-effects model) with treatment as the between groups factor and
time as the within groups factor. Treatment [F (1, 25) = 35.96],
time [F (6.000, 99.00) = 44.83] and treatment x time interaction
[F (6, 99) = 13.23], all p<0.001. Post-hoc Bonferroni
tests between treatment groups at each time point revealed significant
increases in mechanical threshold by BW-031 at 1, 3 and 5 hours post
treatment, p<0.001. N=5-15 male rats per group,
*p<0.05, **p<0.01, ***p<0.001. Bars for
panels a-b represent mean±SEM for each condition, while the
individual data points are displayed as open circles.