Figure 4. BW-031 produces long-lasting analgesia in rat paw CFA injection and surgical paw incision pain models.
a, Hargreaves assay of hindpaw thermal sensitivity in rats after intraplantar injection of Complete Freund’s Adjuvant (CFA) alone (white), 2% QX-314 dissolved in CFA (blue) or 2% BW-031 dissolved in CFA (red). Both QX-314 and BW-031 produce robust thermal analgesia. Two-way repeated measures ANOVA with treatment as the between groups factor and time as the within groups factor. Treatment [F(2, 27)=15.05], time [F(1.980, 53.46)=14.88], and treatment x time interaction [F(4, 54)=6.767], all p<0.001. Post-hoc Tukey’s tests between treatment groups at each time point revealed significant increases in mechanical threshold by BW-031 at 1 and 4 hours post treatment and QX-314 at 1h post treatment. N=10 male rats per group, *p<0.05, **p<0.01, ***p<0.001.b , Von Frey measurements of hindpaw mechanical sensitivity in rats after paw incision and intraplantar injection of vehicle or 2% BW-031. Baseline measurements were made before paw incision. Vehicle or 2% BW-031 was injected 24 hours after the incision. Two-way ANOVA (mixed-effects model) with treatment as the between groups factor and time as the within groups factor. Treatment [F (1, 25) = 35.96], time [F (6.000, 99.00) = 44.83] and treatment x time interaction [F (6, 99) = 13.23], all p<0.001. Post-hoc Bonferroni tests between treatment groups at each time point revealed significant increases in mechanical threshold by BW-031 at 1, 3 and 5 hours post treatment, p<0.001. N=5-15 male rats per group, *p<0.05, **p<0.01, ***p<0.001. Bars for panels a-b represent mean±SEM for each condition, while the individual data points are displayed as open circles.