2. MATERIAL and METHODS
Of 101 children who were hospitalized between 1thApril 2017 and 1th June 2018 in pediatric surgical
intensive care unit due to trauma and 101 other children admitted by
circumcision or incarcerated inguinal hernia were included to this
study. The study was conducted prospectively in our hospital. The study
permission was obtained from the ethics committee of the Ankara Child
Health and Diseases Hematology Oncology Training and Research Hospital
(decision no. 2017/05 dated 27/03/2017). Informed consent form was
obtained from the parents of both groups’ children included to the
study. The cases with trauma were compared with the control group in
terms of thiol-disulfide levels and IMA. On the 2nd and 5th days of
hospitalization, 2 mL of venous blood was taken to the yellow blood tube
(BD Vacutainer plastic SST II tube ®) from children with trauma. Blood
samples collected were centrifugated at 3600 rpm for 10 minutes in the
biochemistry laboratory and 2 cc serum was obtained and stored at -80°C.
After all the samples were collected, all of them were thawed at the
same time and the blood thiol-disulfide parameters were determined by
Erel & Neselioglu with the newly developed automatic measurement method
[7] and the IMA levels were determined by Bar-Or et al. [29]
method via the Roche Hitachi Cobas c501 automatic analyzer. The levels
of native thiol (-SH), total thiol (SH + SS), dynamic disulfide (SS),
dynamic disulfide (SS) / total thiol (SH + SS), albumin and ischemic
modified albumin (IMA) were measured from the sera of patients and
control group.
2.1.Statistical analysis: Statistical analysis was performed
using SPSS 17.0 version (Chicago Inc., 2008). Shapiro-Wilk test was used
to determine normality of the continuous variables. Numerical variables
were controlled by analytical methods. Total thiol, native thiol (SH),
dynamic disulphid (SS) and dynamic disulphid (SS) / total thiol (SS +
SH), albumin and IMA values did not fit the normal distribution. For
comparison, variables were analyzed by Mann-Whitney U and paired
correlation test. p<0.05 was considered significant.