2. MATERIAL and METHODS
Of 101 children who were hospitalized between 1thApril 2017 and 1th June 2018 in pediatric surgical intensive care unit due to trauma and 101 other children admitted by circumcision or incarcerated inguinal hernia were included to this study. The study was conducted prospectively in our hospital. The study permission was obtained from the ethics committee of the Ankara Child Health and Diseases Hematology Oncology Training and Research Hospital (decision no. 2017/05 dated 27/03/2017). Informed consent form was obtained from the parents of both groups’ children included to the study. The cases with trauma were compared with the control group in terms of thiol-disulfide levels and IMA. On the 2nd and 5th days of hospitalization, 2 mL of venous blood was taken to the yellow blood tube (BD Vacutainer plastic SST II tube ®) from children with trauma. Blood samples collected were centrifugated at 3600 rpm for 10 minutes in the biochemistry laboratory and 2 cc serum was obtained and stored at -80°C. After all the samples were collected, all of them were thawed at the same time and the blood thiol-disulfide parameters were determined by Erel & Neselioglu with the newly developed automatic measurement method [7] and the IMA levels were determined by Bar-Or et al. [29] method via the Roche Hitachi Cobas c501 automatic analyzer. The levels of native thiol (-SH), total thiol (SH + SS), dynamic disulfide (SS), dynamic disulfide (SS) / total thiol (SH + SS), albumin and ischemic modified albumin (IMA) were measured from the sera of patients and control group.
2.1.Statistical analysis: Statistical analysis was performed using SPSS 17.0 version (Chicago Inc., 2008). Shapiro-Wilk test was used to determine normality of the continuous variables. Numerical variables were controlled by analytical methods. Total thiol, native thiol (SH), dynamic disulphid (SS) and dynamic disulphid (SS) / total thiol (SS + SH), albumin and IMA values did not fit the normal distribution. For comparison, variables were analyzed by Mann-Whitney U and paired correlation test. p<0.05 was considered significant.