B- and T-cell immunophenotyping
B-cell subpopulations (including CD21low, naïve,
transitional, marginal zone-like, class-switched cells, and
plasmablasts) and T-cell subpopulations (recent thymic emigrants
[RTE], naïve, central memory [CM], effector memory [EM],
effector memory expressing CD45RA [TEMRA], and activated T cells)
were analyzed using antibody-fluorochrome conjugates for
fluorescence-activated cell sorting (FACS) (Supplementary Table 1).