2.11 Data and statistical analyses
Experimental design and statistics were as recommended by theBritish Journal of Pharmacology (Curtis et al. , 2018). All experiments were done using 5 or 8 independent replicates (n = 5 or n = 8). Results are expressed as means ± SEM. All statistical analyses were performed with GraphPad Prism v.7.0 and P < 0.05 was considered to show statistical significance between group means. Confocal microscopy (n = 5), plasma extravasation (n= 8) and nociception experiments (n = 8) were analysed by one-way ANOVA. MS-based plasma peptide quantitation (n= 5), vascular reactivity experiments and mean arterial pressure (MAP, n = 5) and heart rate (HR, n = 5) measurements were analysed by two-way ANOVA. Relative mRNA quantitation (qPCR, n= 5) data were analysed by unpaired t test (parametric test due to normal distribution).