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High-throughput phenotyping-based QTL mapping reveals the genetic architecture of the salt stress tolerance of Brassica napus
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  • Guofang Zhang,
  • Jinzhi Zhou,
  • Yan Peng,
  • Zengdong Tan,
  • Yuting Zhang,
  • Hu Zhao,
  • Dongxu Liu,
  • Xiao Liu,
  • Long Li,
  • Liangqian Yu,
  • Cheng Jin,
  • Shuai Fang,
  • Jiawei Shi,
  • Zengdong Geng,
  • Shanjing Yang,
  • Guoxing Chen,
  • Kede Liu,
  • Qingyong Yang,
  • Hui Feng,
  • Liang Guo,
  • Wanneng Yang
Guofang Zhang
Huazhong Agricultural University
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Jinzhi Zhou
Huazhong Agricultural University
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Yan Peng
Huazhong Agricultural University
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Zengdong Tan
Huazhong Agricultural University
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Yuting Zhang
Huazhong Agricultural University
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Hu Zhao
Huazhong Agricultural University
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Dongxu Liu
Huazhong Agricultural University
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Xiao Liu
Huazhong Agricultural University
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Long Li
Huazhong Agricultural University
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Liangqian Yu
Huazhong Agricultural University
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Cheng Jin
Huazhong Agricultural University
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Shuai Fang
Huazhong Agricultural University
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Jiawei Shi
Huazhong Agricultural University
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Zengdong Geng
Huazhong Agricultural University
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Shanjing Yang
Huazhong Agricultural University
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Guoxing Chen
Huazhong Agricultural University
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Kede Liu
Huazhong Agricultural University
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Qingyong Yang
Huazhong Agricultural University
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Hui Feng
Huazhong Agricultural University
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Liang Guo
Huazhong Agricultural University
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Wanneng Yang
Huazhong Agricultural University
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Abstract

Salt stress is a major limiting factor that severely affects the survival and growth of crops. It is important to understand the salt tolerance ability of Brassica napus and explore the underlying related genetic resources. We used a high-throughput phenotyping platform to quantify 2,111 image-based traits (i-traits) of a natural population under 3 different salt stress conditions and an intervarietal substitution line (ISL) population under 9 different stress conditions to monitor and evaluate the salt stress tolerance of B. napus over time. We finally identified 928 high-quality i-traits associated with the salt stress tolerance of B. napus. Moreover, we mapped the salt stress-related loci in the natural population via a genome-wide association study (GWAS) and performed a linkage analysis associated with the ISL population, respectively. The results revealed 234 candidate genes associated with salt stress response, and two novel candidate genes, BnCKX5 and BnERF3, were experimentally verified to regulate the salt stress tolerance of B. napus. This study demonstrates the feasibility of using high-throughput phenotyping-based QTL mapping to accurately and comprehensively quantify i-traits associated with B. napus. The mapped loci could be used for genomics-assisted breeding to genetically improve the salt stress tolerance of B. napus.

Peer review status:UNDER REVIEW

03 Sep 2021Submitted to Plant, Cell & Environment
03 Sep 2021Assigned to Editor
03 Sep 2021Submission Checks Completed
19 Sep 2021Reviewer(s) Assigned