Fig.5 . Diluted blood cells focusing. (A) Blood cells remain well focused at the outer wall at higher blood dilutions (1:10 and 1:5). As the hematocrit content increases (25% in case of 1:2 dilution), blood cells begin to spread away from the outer wall due to an increase in particle-particle interaction. Blood dilution is thus important to achieve good focusing and separation of blood cells. Scale bar: 100 µm. (B) Particle separation from diluted blood. Bar graph showing the separation of 1 µm particles with an efficiency of 97%, 93%, and 83% from 1:10, 1:5, and 1:2 diluted blood, respectively.