Chip characterization
A two-turn spiral channel with a rectangular cross-sectional geometry was first designed and tested by Bhagat et al. 58 to isolate CTCs from blood. A similar design was used to study the behavior of particles of different sizes in our work. The design is shown in Fig. 1 in the center. The microchannel had a width of w = 500 µm and a height of h = 50 µm. The aspect ratio, defined as width/height (w/h) was 10. The blockage ratio, which is defined as a/h, where ‘a’ is the diameter of the particle and ‘h’ is the channel height, ranged from 0.02 to 0.14.