MdNup62 interacts with MdHSFs
We cloned parts of the MdHSF s
(MdHSFA1a/b/d/e andMdHSFA9a/b ) independently into the pGADT7 vector and then
cotransformed each with MdNUP62508–613-pGBKT7 .MdNup62 interacted with these MdHSF s (Figure 7a).Additionally, we used MdHSFA9b in pull-down assays. The
recombinant MdNup62-HIS fusion protein was purified withMdHSFA9b-GST , but not with GST alone (Figure 7b). The split-LUC
complementation assay revealed that the co-expression ofMdNup62-NLUC with MdHSFA1d -CLUC or MdHSFA9b -CLUC
resulted in a higher LUC activity than the other combinations (Figure
7c–e). These results confirmed the interaction between MdNup62and both MdHSFA1D and MdHSFA9b .