Heat map, protein alignment, and phylogenetic analysis
Based on RNA-seq data of our laboratory, the heat map of apple different tissues was constructed using MeV (Multiple Experiment Viewer) software. A protein sequence alignment of Nup62 from six Rosaceae plants was performed using DNAMAN software. The Nup62 protein sequences were obtained from the GDR database (https://www.rosaceae.org/). The phylogenetic tree was constructed using MEGA-X software.