Figure 5. Changes in the level of accumulated ROS and activities
of ROS-scavenging enzymes in OE-MdNup62 and WT Arabidopsis leaves
under heat-stress conditions.
(a) In situ accumulations of superoxide radicals (O2−)
before (upper panels) and after (lower panels) heat treatment as
revealed by nitro blue tetrazolium staining. (b) and (c) Quantitative
measurement of H2O2 and malondialdehyde
concentrations in Arabidopsis leaves treated with and without the high
temperature. (d)–(f) Activities of superoxide dismutase (SOD),
peroxidase (POD), and catalase (CAT) at 6 h after the heat treatment.
Each sample was analysed with three biological replicates, each
comprising three technical replicates. Means followed by different
lowercase letters are significantly different at the 0.05 level.