MdNup62-interacting protein screening
To further reveal the function of MdNup62 , we conducted a Y2H sieve library experiment using a MdNup62 truncated body (MdNup62508–613-pGBKT7 ) that is not self-activated. We identified 62 putative MdNup62 -interacting proteins (Table S3). Some transcription factors were identified, such as HSFs (MdHSFA1d , MdHSFA1e , MdHSFA9 , MdHSF30 ,MdHSF1 , and MdHSF8 ), as well as MdMYB21 ,MdMYC2 , MdGATA11 , and MdBAK1 . In addition, some enzymes and other functional genes were found. Because transcription factors that have transcriptional regulatory functions must be transported into the nucleus, and because MdNup62 has regulatory effects on the transport of the proteins, we hypothesized thatMdNup62 interacts with these MdHSF s and controls their transport.