Figure 9. MdHSFA9b promotes flowering in Arabidopsis.
(a) Phenotype of the MdHSFA9b -overexpression Arabidopsis line for flowering time. Bar = 2 cm. (b) Statistical analysis of rosette leaves of Arabidopsis thaliana during bolting. Asterisks denote significant differences as determined by a t-test (*P < 0.05). (c) Semi-quantitative RT-PCR analysis of MdHSFA9b expression in Arabidopsis samples. (d) qRT-PCR analysis of MdHSFA9b expression in Arabidopsis samples. Asterisks denote significant differences as determined by a t-test (**P < 0.01). (e) Relative expression levels of flowering genes (AtFT , AtLFY , AtSOC1 , andAtAP1 ) in WT and MdHSFA9b -overexpression lines. Each sample was analysed with three biological replicates, each comprising three technical replicates. Asterisks denote significant differences as determined by a t-test (*P < 0.05).
Figure 10. MdHSFA9b enhanced high-temperature resistance in Arabidopsis.
(a) Phenotype of the MdHSFA9b -overexpression Arabidopsis line for high-temperature resistance. Bar = 2 cm. (b) Survival rates of WT andMdHSFA9b -overexpression Arabidopsis lines after the high-temperature treatment. Asterisks denote significant differences as determined by a t-test (**P < 0.01). (c) Relative expression levels of high-temperature resistance-related genes (AtHSP101 ,AtHSP22.0-ER , AtHSP21 , and AtHSP70T-2 ) in WT andMdHSFA9b -overexpression lines at the normal temperature (22°C) and 1 h after exposure to the high-temperature (45°C) treatment. Each sample was analysed with three biological replicates, each comprising three technical replicates. Asterisks denote significant differences as determined by a t-test (*P < 0.05).