Overexpression of MdHSFA9b and MdHSFA1d enhances high-temperature resistance
To study the high-temperature resistance phenotypes of MdHSFA9band MdHSFA1d , we also exposed OE-MdHSFA9b and OE-MdHSFA1d transgenic plants, respectively, to high-temperature (45°C) conditions (Figures 10a and S4a). The survival rates of OE-MdHSFA9b and OE-MdHSFA1d lines were significantly greater than that of WT (Figures 10b and S4b). Consistently, the ROS accumulation in leaves decreased in transgenic plants after the high-temperature treatment (Figure S5a, b). We also performed a qRT-PCR analysis of A. thaliana HSPs (AtHSP101 ,AtHSP22-ER , AtHSP21.0 , and AtHSP70T-2 ) (Figures 10c and S4c), and their expression levels in transgenic A. thalianaincreased under high-temperature conditions compared with under normal growth conditions. These results indicated that MdHSFA9b andMdHSFA1d enhance plant high-temperature resistance.