Figure 6. MdNup62 reduced high-temperature resistance in tomato.
(a) Phenotype of the MdNup62 -overexpression tomato line for high-temperature resistance. Bar = 5 cm. (b) Survival rates of WT andMdNup62 -overexpression tomato lines after the high-temperature treatment. Asterisks denote significant differences as determined by a t-test (**P < 0.01). (c) qRT-PCR analysis of MdNup62expression levels in tomato samples. Asterisks denote significant differences as determined by a t-test (**P < 0.01). (d) Genomic PCR analysis of MdNup62 transgenic tomato lines. (e) Relative expression levels of high-temperature resistance-related genes (SlHSP101 , SlHSP22.0-ER , SlHSP21 , andSlHSP70T-2 ) in WT and MdNup62 -overexpression lines at the normal temperature (22°C) and 1 h after exposure to the high-temperature (45°C) treatment. Each sample was analysed with three biological replicates, each comprising three technical replicates. Asterisks denote significant differences as determined by a t-test (*P < 0.05).