Subcellular localization
The open reading frames (ORFs) of the MdNup62 , MdHSFA1d ,
and MdHSFA9b genes were inserted independently into the
pCAMBIA2300-EGFP vector to generate the 35S::MdNup62 -EGFP,
35S::MdHSFA1d -EGFP, and 35S::MdHSFA9b -EGFP recombinant
plasmids, respectively. These recombinant plasmids were inserted
independently into Agrobacterium tumefaciens strain GV3101 cells.
The GV3101 cells containing these recombinant plasmids were then
infiltrated into tobacco leaves. GV3101 cells containing the
pCAMBIA2300-EGFP vector (35S::EGFP) served as the control. After an
additional 3 days of growth in the dark, green fluorescent protein (GFP)
signals in transformed tobacco leaves were detected using a Leica TCS
SP8 SR Laser Scanning Confocal Microscope (Leica, Germany).The primers
used are listed in Table S5.