Heat-tolerance assays
The ‘Fuji’ plants at 30 days after propagation were used for the 45°C heat treatment. We collected leaf samples before and at 1, 3, and 6 h after the treatment. The samples were immediately frozen in liquid nitrogen and stored at −80°C for later use.
Two-week-old transgenic Arabidopsis and 3-week-old transgenic tomato were used for the heat treatment in an artificial climate chamber. OE-MdNup62 A. thaliana lines were subjected to 45°C for 12 h, and OE-MdHSFA9b and OE-MdHSFA1d A. thalianalines were subjected to 45°C for 16 h. OE-MdNup62 tomato lines were subjected to 45°C for 14 h.