Figure 8. Subcellular localization and expression analyses ofMdHSFA9b and MdHSFA1d.
(a) Phylogenetic analysis of HSFs in Malus and Arabidopsis.(b) Tissue specific expression patterns of apple MdHSFs by RNA-seq. The full names of the different abbreviations are as follows, ‘Nagafu No.2’ long branches flower buds (FLB), ‘Nagafu No.2’ short branches flower buds (FSB), ‘Nagafu No.2’ axillary buds (FYB), ‘Qinguan’ axillary buds (QYB), ‘Nagafu No.2’ fruit (FR), ‘Yanfu No.3’ stem tip (YF3J), ‘Yanfu No.6’ stem tip (YF6J), and ‘M9-T337’ root (T337R). Each number after the abbreviation represents a biological repetition. (c) Subcellular localizations of MdHSFA9b and MdHSFA1d . The upper panel shows 35S::EGFP, the middle panel shows 35S::MdHSFA9b -EGFP, and the lower panel shows 35S::MdHSFA1d -EGFP. (d) Analyses of MdHSFA9b andMdHSFA1d expression levels in diverse apple ‘Nagafu No. 2’ tissues and in different flower bud developmental stages of ‘Nagafu No. 2’. Each sample was analysed with three biological replicates, each comprising three technical replicates. Means followed by different lowercase letters are significantly different at the 0.05 level.