Overexpression of MdHSFA9b and MdHSFA1d promotes
flowering
To verify the flowering phenotype of HSFs, we performed
Agrobacterium-mediated genetic transformations of MdHSFA9b andMdHSFA1d into A. thaliana . Like OE-MdNup62 ,
OE-MdHSFA9b and OE-MdHSFA1d lines flowered significantly
earlier than WT (Figures 9a and S3a). Additionally, they also had
significantly fewer rosette leaves than WT during bolting (Figures 9b
and S3b). We also performed genomic PCR (Figure S2b, c),
semi-quantitative RT-PCR (Figures 9c and S3c), and qRT-PCR (Figures 9d
and S3d) to confirm the presence of the transgene in the
OE-MdHSFA9b and OE-MdHSFA1d lines. The transcript levels
of AtFT , AtLFY , and AtSOC1 were significantly
increased in OE-MdHSFA9b and OE-MdHSFA1d lines compared
with WT (Figures 9e and S3e).