Subcellular localization
The open reading frames (ORFs) of the MdNup62 , MdHSFA1d , and MdHSFA9b genes were inserted independently into the pCAMBIA2300-EGFP vector to generate the 35S::MdNup62 -EGFP, 35S::MdHSFA1d -EGFP, and 35S::MdHSFA9b -EGFP recombinant plasmids, respectively. These recombinant plasmids were inserted independently into Agrobacterium tumefaciens strain GV3101 cells. The GV3101 cells containing these recombinant plasmids were then infiltrated into tobacco leaves. GV3101 cells containing the pCAMBIA2300-EGFP vector (35S::EGFP) served as the control. After an additional 3 days of growth in the dark, green fluorescent protein (GFP) signals in transformed tobacco leaves were detected using a Leica TCS SP8 SR Laser Scanning Confocal Microscope (Leica, Germany).The primers used are listed in Table S5.