Figure 7. MdNup62 interacts with MdHSFs.
(a) Interactions between MdNup62508−613 andMdHSF s (MdHSFA9a/b and MdHSFA1a/b/d/e ) in Y2H
assays. The MdNup62508−613 truncated sequence
was cloned into pGBKT7, whereas MdHSFA s (MdHSFA9a/b andMdHSFA1Da/b/d/e ) were cloned independently into the pGADT7
vector. Empty pGADT7 plus MdNup62508−613 -pGBKT7
was used as the control. (b) Interactions between MdNup62 andMdHSFA9b in the pull-down assay. Western blotting with a GST
antibody revealed that MdNup62-HIS was pulled down byMdHSFA9b-GST . (c)–(e) Interactions between MdNup62 and
both MdHSFA9b and MdHSFA1d in a luciferase (LUC)
complementation experiment. Empty NLUC and empty CLUC,MdNup62 -NLUC plus empty CLUC, empty NLUC plus MdHSFA9b ,
and MdHSFA1d -CLUC were used as controls. The LUC complementation
experiment was repeated three times, with consistent results. Asterisks
denote significant differences as determined by t-tests (** P
< 0.01).