Figure 5. Changes in the level of accumulated ROS and activities of ROS-scavenging enzymes in OE-MdNup62 and WT Arabidopsis leaves under heat-stress conditions.
(a) In situ accumulations of superoxide radicals (O2−) before (upper panels) and after (lower panels) heat treatment as revealed by nitro blue tetrazolium staining. (b) and (c) Quantitative measurement of H2O2 and malondialdehyde concentrations in Arabidopsis leaves treated with and without the high temperature. (d)–(f) Activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) at 6 h after the heat treatment. Each sample was analysed with three biological replicates, each comprising three technical replicates. Means followed by different lowercase letters are significantly different at the 0.05 level.