Overexpression of MdNup62 promotes flowering
To confirm MdNup62 ’s role in flowering, we performed an Agrobacterium-mediated genetic transformation of MdNup62 intoA. thaliana . We found that OE-MdNup62 lines flowered significantly earlier than WT (Figure 3a). Additionally, OE-MdNup62 lines had significantly fewer rosette leaves than WT during bolting (Figure 3b). The presence of the transgene in OE-MdNup62 lines was confirmed using genomic PCR (Figure S2a), semi-quantitative RT-PCR (Figure 3c), and qRT-PCR (Figure 3d). The transcript levels of flowering-related genes were analysed by qRT-PCR (Figure 3e). The expression levels of AtFT , AtLFY , andAtAP1 significantly increased in OE-MdNup62 lines compared with WT. This demonstrated that the overexpression of MdNup62promoted flowering in Arabidopsis.