Figure 2. Identification and analysis of MdNup62.
(a) Phylogenetic analysis of Rosaceae Nup62 . (b) The conservative
domain of Rosaceae Nup62 . (c) Subcellular localization ofMdNup62 . The upper panel shows 35S::EGFP, and the lower panel
shows 35S::MdNup62-EGFP. (d) and (e) Analyses of MdNup62expression levels in diverse ‘Nagafu No. 2’ apple tissues (d) and in
different flower bud developmental stages of ‘Nagafu No. 2’ (e). (f) The
phenotype of
‘Nagafu
No. 2’ tissue-cultured seedlings (upper panels) and the in situ
accumulation of superoxide radical (O2−) at 0, 1, 3,
and 6 h under heat treatment conditions (lower panels). Bar = 1 cm. (g)MdNup62 expression levels in ‘Nagafu No. 2’ tissue-cultured
seedling leaves at 0, 1, 3, and 6 h under heat-treatment conditions.
Each sample was analysed with three biological replicates, each
comprising three technical replicates. Means followed by different
lowercase letters are significantly different at the 0.05 level.