MdNup62-interacting protein screening
To further reveal the function of MdNup62 , we conducted a Y2H
sieve library experiment using a MdNup62 truncated body
(MdNup62508–613-pGBKT7 ) that is not
self-activated. We identified 62 putative MdNup62 -interacting
proteins (Table S3). Some transcription factors were identified, such as
HSFs (MdHSFA1d , MdHSFA1e , MdHSFA9 , MdHSF30 ,MdHSF1 , and MdHSF8 ), as well as MdMYB21 ,MdMYC2 , MdGATA11 , and MdBAK1 . In addition, some
enzymes and other functional genes were found. Because transcription
factors that have transcriptional regulatory functions must be
transported into the nucleus, and because MdNup62 has regulatory
effects on the transport of the proteins, we hypothesized thatMdNup62 interacts with these MdHSF s and controls their
transport.