Figure S4. MdHSFA1d enhanced high-temperature resistance in Arabidopsis.
(a) Phenotype of the MdHSFA1d -overexpression Arabidopsis line for high-temperature resistance. Bar = 2 cm. (b) Survival rates of WT andMdHSFA1d -overexpression Arabidopsis lines after the high-temperature treatment. Asterisks denote significant differences as determined by a t-test (**P < 0.01). (c) Relative expression levels of high-temperature resistance-related genes (AtHSP101 ,AtHSP22.0-ER , AtHSP21 , and AtHSP70T-2 ) in WT andMdHSFA1d -overexpression lines at the normal temperature (22°C) and 1 h after exposure to the high-temperature (37°C) treatment. Each sample was analysed with three biological replicates, each comprising three technical replicates. Asterisks denote significant differences as determined by a t-test (**P < 0.01).
Figure S5. Changes in the levels of accumulated ROS in Arabidopsis leaves under heat-stress conditions . (a) and (b) In situ accumulations of MdHSFA9b (a), MdHSFA1d (b), and superoxide radicals (O2−) before (upper panels) and after (lower panels) the heat treatment as revealed by nitro blue tetrazolium staining. Bar = 1 cm.
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