Yeast two-hybrid (Y2H) assay
The MdNup62 508–613 truncated sequence was
cloned into the pGBKT7 vector to generate theMdNup62 508–613-pGBKT7 recombinant plasmid. The
MdHSFAs’ ORFs were inserted individually into the pGADT7 vector to
generate the MdHSFAs-pGADT7 recombinant plasmids. The recombinant
plasmids were inserted into Gold Yeast Two-Hybrid cells, which were then
grown on a selective medium. The primers used are listed in Table S5.