Figure S3. MdHSFA1d promotes flowering in Arabidopsis.
(a) Phenotype of the MdHSFA1d -overexpression Arabidopsis line for
flowering time. Bar = 2 cm. (b) Statistical analysis of rosette leaves
of Arabidopsis thaliana during bolting. Asterisks denote
significant differences as determined by a t-test (*P < 0.05).
(c) Semi-quantitative RT-PCR analysis of MdHSFA1d expression in
Arabidopsis samples. (d) qRT-PCR analysis of MdHSFA1d expression
in Arabidopsis samples. Asterisks denote significant differences as
determined by a t-test (*P < 0.01). (e) Relative expression
levels of flowering genes (AtFT , AtLFY , AtSOC1 , andAtAP1 ) in WT and MdHSFA1d -overexpression lines. Each
sample was analysed with three biological replicates, each comprising
three technical replicates. Asterisks denote significant differences as
determined by a t-test (*P < 0.05).