Overexpression of MdNup62 promotes flowering
To confirm MdNup62 ’s role in flowering, we performed an
Agrobacterium-mediated genetic transformation of MdNup62 intoA. thaliana . We found that OE-MdNup62 lines flowered
significantly earlier than WT (Figure 3a). Additionally,
OE-MdNup62 lines had significantly fewer rosette leaves than WT
during bolting (Figure 3b). The presence of the transgene in
OE-MdNup62 lines was confirmed using genomic PCR (Figure S2a),
semi-quantitative RT-PCR (Figure 3c), and qRT-PCR (Figure 3d). The
transcript levels of flowering-related genes were analysed by qRT-PCR
(Figure 3e). The expression levels of AtFT , AtLFY , andAtAP1 significantly increased in OE-MdNup62 lines compared
with WT. This demonstrated that the overexpression of MdNup62promoted flowering in Arabidopsis.