Experimental Animals
16 healthy male Wistar albino rats weighing between 210-304 grams that
were shown to have healthy outer ear canals and tympanic membrane in
otoscopic examination were used in our study. All of the experimental
animals were housed in appropriate cages under standard environmental
conditions (room temperature
22oC-24oC, %50 relative humidity
and 12 hour periods of light-dark). The animals could access water and
traditional laboratory diet until they were sacrificed.