Conclusion
In the experimental cholesteatoma model created in rats, no statistical
significance was observed, indicating that Mesna, which was applied
intratympanically, completely prevented the formation of cholesteatoma.
However, it was found that the prevalence of cholesteatoma formation was
microscopically less in the experimental group.
Keywords: Mesna, cholesteatoma, fibrosis, otitis media,
intratympanic
What’s known?
Mesna is a synthetic sulfur compound that carries a thiol
group. It breaks the disulfide bonds in a polypeptide chain with
mucolysis
The matrix of the cholesteatoma or squamous epithelial is made
of keratin. Keratin is a protein that has disulfide bonds.
What’s new?
Mesna can be used to treatment of cholesteatoma due to its
mucolysis property.
Backgraund
Various studies have been published on the development of otitis media
and cholesteatoma after the intratympanic application of chemicals on
laboratory animals.1-3 In the 1980’s, it has been seen
that the eye and ear drop called Cortisporin caused inflammatory changes
and the formation of cholesteatoma in the middle ear.4It was shown that this effect of Cortisporin was due to the %10
propylene glycol used as a solvent. 4 In the following
years propylene glycol has been used in experimental studies in the
development of otitis media and cholesteatoma because of its
inflammatory property for the ear.5-7 Sodium 2 -
mercaptoethanesulfonate (C2 H 5 NaO 3S 2, Mesna ) is a synthetic sulfur
compound that carries a thiol group. It breaks the disulfide bonds in a
polypeptide chain with mucolysis. The matrix of the cholesteatoma or
squamous epithelial is made of keratin. Keratin is a protein that has
disulfide bonds. Mesna can be used to ease the dissection of the tissue
layers in the surgery of cholesteatoma due to its mucolysis
property.8 In the studies conducted, it has been
reported that Mesna has no side effects or hazard on hearing subsequent
to being applied to the middle ear cavity.9-10 For
this reason we planned to investigate the effect of Mesna on the
cholesteatoma and otitis media created in the middle ear cavity of
experimental animals by propylene glycol. We aimed to show the presence
of keratinized epithelium in the middle ear, the inflammatory changes in
the middle ear mucosa and the changes in the morphology of the tympanic
membrane through histopathological evaluation.
Methods
The study was conducted in line with the Guide for the Care and Use of
Laboratory Animals issued by the National Institutes of Health,
Commission on Life Sciences, and National Research
Council.11 The study protocol was approved by the
ethical committee of our institution (document no. 2013/5).