Table 3 F ST estimates between demes I and II of Lakes Bunnersjöarna. For Pool-seq data Nei´s (1973)F ST was used with window size = 5,000 bp, read depth = 20X – 150X and fraction depth covered (the fraction to which windows were covered with data) ≥ 0.8. For the 96 SNP fluidigm assay, 77 polymorphic loci were used and for the allozymes 7 and 8 polymorphic loci were used (excluding and including LDH-1 , respectively; data from Ryman et al. 1979 and unpublished data). FSTwas computed using Nei’s (1973) and Weir and Cockerham’s (1984) approaches.