2 MATERIAL AND METHODS
We used tissues from the material collected in 1975
(Allendorf et al., 1976;
Ryman et al., 1979) that have been stored
at -30°C. We first applied a 96 SNP fluidigm array to verify that the
existence of the sympatric populations can be detected by markers other
than allozymes. Next, we sequenced whole genomes from several pooled
individuals (Pool-seq; Kofler, Langmüller,
Nouhaud, Otte, & Schlötterer, 2016) as well as from two separate
individuals of each deme.