Figure 2. The analysis of N-terminal and C-terminal to
XynA. (A) Structural superposition of XynA, SlXyn10A (PDB code
1V0L), and TsXy1A (PDB code 3W25). (B) Protein thermal shift assay of
XynA and XynA_ΔN37. (C) Relative activities of the XynA_ΔN37 in the
hydrolysis of BWX, WAX and barley β-glucan respectively. * indicates P
<0.05, **** indicates P <0.0001. (D) The hydrogen
bonding interaction between the N-terminal and GH10 domains. The red
dashed line indicates hydrogen bonding interaction. (E) The hydrogen
bonding interaction between the C-terminal and GH10 domains. The red
dotted line represents hydrogen bonds. The criterion for hydrogen bond
judgment is that the distance between the acceptor and donor atoms
should be less than 4 Å.