Figure 3. Multiple sequence alignment and structure analyses of XynA. Multiple sequence alignment of XynA (QCO69162) with CbXyn10C (ACM60945) from C. bescii (Chu et al., 2017); SlXyn10A (AAC26525) from S. lividans (Ducros et al., 2000b); BsXynA (AHH02587) fromBacillus sp.(Zhou et al., 2014b); TmXynB (AAD35164) from T. maritima (Ihsanawati et al., 2005); and TsXy1A (AFK86466) from T. saccharolyticum (Han et al., 2013). The red background color represents strictly conserved residues, and the red font represents the substitution of conserved residues. The pink five-pointed star indicates the two key catalytic residues of XynA, and the original secondary structure of XynA is shown above the sequence alignment. The black squares represent the residues that interact with the substrate in CbXyn10C, and the white squares represent the residues that interact with the substrate in TsXy1A. The triangles indicate the residues of XynA and BsXynA that differ in the GH10 domain.