Figure 2. The analysis of N-terminal and C-terminal to XynA. (A) Structural superposition of XynA, SlXyn10A (PDB code 1V0L), and TsXy1A (PDB code 3W25). (B) Protein thermal shift assay of XynA and XynA_ΔN37. (C) Relative activities of the XynA_ΔN37 in the hydrolysis of BWX, WAX and barley β-glucan respectively. * indicates P <0.05, **** indicates P <0.0001. (D) The hydrogen bonding interaction between the N-terminal and GH10 domains. The red dashed line indicates hydrogen bonding interaction. (E) The hydrogen bonding interaction between the C-terminal and GH10 domains. The red dotted line represents hydrogen bonds. The criterion for hydrogen bond judgment is that the distance between the acceptor and donor atoms should be less than 4 Å.