Materials and Methods
In our study, the kidney resection materials that were operated between
January 2010 and December 2019 with the pre-diagnosis of kidney tumor
and diagnosed with WT in our department were examined. Twenty-four
patients diagnosed with WT, who did not receive chemo-radiotherapy
before surgery, and whose paraffin blocks could be accessed were
included in the study. Since the paraffin blocks of 3 patients could not
be reached, these patients were not included in the study. The paraffin
blocks and glasses of the patients were taken out of the archive and
sections were taken again and examined under the light microscope. Four
micron-thick sections were taken from the blocks containing the most
intensive tumor tissue and placed on the charged glass slides. After
keeping at 70°C for 15 minutes, they were placed in the automated
immunohistochemistry staining device (Ventana, Roche, USA). After the
slides were subjected to deparaffinization and dehydration processes,
respectively, they were processed with ULTRA Cell Conditioning Solution,
hydrogen peroxidase, and COX-2 antibodies (Nova Castra, Leica,
Newcastle, United Kingdom). Immunhistochemical study was performed on
the immunohistochemically selected blocks using the COX-2 antibody.
Significant stoplasmic and luminal staining of ≥10% in tumoral areas
was considered positive 7.
Results were compared with renal pelvis invasion, renal sinus soft
tissue invasion, renal sinus lymphovascular invasion, renal vein
invasion, renal capsular invasion, perirenal adipose tissue invasion,
macroscopic tumor diameter, presence of anaplasia, presence of
nephrogenic residue, lymphovascular invasion, TNM stage, and survival,
which are significant prognostic parameters. These parameters could not
be compared since the lymph node dissection was not performed and the
Gerota’s fascia was not sent in most of the patients.