Sequence Data that never made it

Over the past several years our lab has experimented with a variety of high-throughput sequencing platforms with the ultimate goal of addressing important scientific questions. On a few occasions we have been successful. Some of our early work included Roche 454 GS-FLX pyrosequencing used to examine phenotypic plasticity in lake trout (FREDERICK 2010), identify single nucleotide polymorphisms in salmon (E. 2011) and develop genomic resources for Pacific herring (B. 2012).

  • Morera D, Roher N, Ribas L, Balasch JC, Doñate C, Callol A, Boltaña A, Roberts SB, Goetz G, Goetz FW, Mackenzie SA. (2011) RNA-Seq reveals an integrated immune response in nucleated erythrocytes. PLoS ONE 6(10): e26998. doi:10.1371/journal.pone.0026998

  • Gavery MR* and Roberts SB. (2012) Characterizing short read sequencing for gene discovery and RNA-Seq analysis in Crassostrea gigas. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 7:2 94-99 June 2012. doi:10.1016/j.cbd.2011.12.003

  • Burge CA, Douglas N, Conti-Jerpe I, Weil E, Roberts SB, Friedman CS, CD Harvell. (2012) Friend or foe: the association of Labyrinthulomycetes with the Caribbean sea fan, Gorgonia ventalina. Diseases of Aquatic Organisms. 101:1-12 doi:10.3354/dao02487

  • Gavery MR, Roberts SB. (2013) Predominant intragenic methylation is associated with gene expression characteristics in a bivalve mollusc. PeerJ 1:e215 doi:10.7717/peerj.215

However there are a number of efforts that have failed early on (ie poor read quality) or just never gained enough momentum to see the light of the peer-review system. This paper is a final attempt to share these datasets in a manner where they have the potenial, however slim, to be useful to someone. We will not be providing fluffy background or in-depth over-analysis but strive to offer a sound data description. This current work will be organized by target species, which will often correspond to project.

Species include

  • Vibrio tubiashii
  • Olympia oyster (Ostrea lurida)
  • Yellow Perch (Perca flavescens)
  • Pacific herring (Clupea pallasii)
  • Abalone / Withering Syndrome (WS)
  • Hard clam (Mercenaria mercenaria)