Morpholino nucleotides specifically blocked the splicing or
translation of rapgef1 in zebrafish
RAPGEF1 is highly conserved through a multitude of species including
zebrafish (Danio rerio ). The homologous gene of humanRAPGEF1 in zebrafish is rapgef1a (ENSDART00000139947.3).
To simulate the function of human RAPGEF1 , we knocked down the
expression of zebrafish rapgef1a by two morpholino nucleotides,
targeting the proper splicing (E2I2-MO) and translation (ATG-MO) ofrapgef1a , respectively (Figure 3 (A)). The effectiveness of the
E2I2-MO was confirmed by testing the presence of skipped exon 2 in the
transcripts of morpholino-injected embryos 3 days after fertilization
(Figure 3 (B)).