Zebrafish Care and Maintenance
Adult wild-type AB strain zebrafish were maintained at 28.5 ℃ on a 14 h light/10 h dark cycle (Westerfield, 2000). Five to six pairs of zebrafish were set up for nature mating every time. On average, 200–300 embryos were generated. Embryos were maintained at 28.5 ℃ in fish water (0.2% Instant Ocean Salt in deionized water). The embryos were washed and staged according to (Kimmel, Ballard, Kimmel, Ullmann, & Schilling, 1995). The establishment and characterization of hb9-EGFP andfli1a-EGFP transgenic lines has been described elsewhere (Kanungo, Lantz, & Paule, 2011; Lawson & Weinstein, 2002). The zebrafish facility is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International.