Measurement of SARS-CoV-2-Spike-specific IgG in Plasma
We next determined the levels of SARS-CoV-2 Spike IgG
using CoVS1-RBD ELISA kit (Ray
Biotech, GA) per the manufacture’s manual. Briefly, the 96 well plates
coated with the SARS-CoV-2 S1 RBD protein were incubated with plasma
followed by biotinylated anti-human IgG. After washing, HRP-conjugated
streptavidin was added, and spike-specific IgG was quantitated by OD450
nm reading.